The dynamical behavior of a model for body fluids in response to an external electric field is computationally investigated for communication frequencies. The effect of an applied potential difference between two electrodes in a saline solution containing a rodlike macromolecule is studied by solving the Poisson and ion continuity equations simultaneously using the finite element method (FEM). ...

BSA solution and the buffer used as a solvent, A is the area of the V = f(T) plot obtained for a given step of the considered thermal denaturation process and a is the part of this surface corresponding to a given temperature T. In this study some conformational changes induced by thermal denaturation of BSA macromolecule were evidenced, the temperatures of transition as well as the # G values ...

PURPOSE To investigate whether diffusion magnetic resonance spectroscopy (MRS) can detect the extracellular matrix (ECM) degradation during intervertebral disc degeneration (IVDD) by the increased mobility of ECM macromolecules such as proteoglycans and collagens. METHODS Fresh bovine intervertebral discs were injected with papain solution to induce ECM degradation. The apparent diffusion coe...

The conformational ensemble of a macromolecule is the complete description of the macromolecule's solution structures and can reveal important aspects of macromolecular folding, recognition, and function. However, most experimental approaches determine an average or predominant structure, or follow transitions between states that each can only be described by an average structure. Ensembles hav...

SAITO, K. Effects of Diquat on Cell Growth; and Macromolecule Synthesis in Cultured Pneumocytes. Tohoku J. exp. Med., 1986, 148 (1), 35-39 The effects of diquat (DQ) on cell growth and macromolecule synthesis were investigated in cultured rat, feline and human. pneumocytes. DQ at 10-3 M and 10-5 M showed timeand dose-dependent inhibition of cell growth for 7 days. The incorporation of 3H-thymid...

PURPOSE Pharmacologic vitreolysis is a promising new therapy to improve vitreoretinal surgery and, ultimately, prevent disease by mitigating the contribution of vitreous to retinopathy. The mechanism of action of the agents being developed for pharmacologic vitreolysis remains unclear. The experiments in this thesis test the hypothesis that pharmacologic vitreolysis agents break down vitreous m...

Here, we present a versatile method for detecting human tumor xenografts in vivo, based on the enhanced permeability and retention (EPR) effect, using near-infrared (NIR) fluorochrome-conjugated macromolecule probes. Bovine serum albumin (BSA) and two immunoglobulins-an anti-human leukocyte antigen (HLA) monoclonal antibody and isotype control IgG2a-were labeled with XenoLight CF770 fluorochrom...

The extracellular matrix (ECM) may contribute to the drug resistance of a solid tumor by preventing the penetration of therapeutic agents. We measured differences in interstitial resistance to macromolecule (IgG) motion in four tumor types and found an unexpected correspondence between transport resistance and the mechanical stiffness. The interstitial diffusion coefficient of IgG was measured ...

Unsupervised machine learning is applied to study the disordered aggregates of a model sequence defined macromolecule. Using these learned collective variables provides new insight into both structure and kinetics aggregates.