BACKGROUND Determination of vancomycin minimum inhibitory concentration (MIC) can influence the agent used to treat methicillin-resistant Staphylococcus aureus (MRSA) infection. We studied diagnostic accuracy using E-test and VITEK(®) 2 against a gold standard broth microdilution (BMD) methodology, the correlation between methods, and associations between vancomycin MIC and MRSA phenotype from ...

A microdilution assay using Alamar Introduction Blue, a colorimetric indicator, was compared with the NCCLS macrodilution broth assay for voriVoriconazole is a new triazole agent that appears to have a broader spectrum of antifungal activity conazole, fluconazole, and itraconazole against Candida albicans, Candida glabrata, and Candida krusei. than the parent compound, fluconazole [1–6]. Prior ...

Standard broth microdilution (with and without bovine serum albumin [BSA] supplementation), tube dilution, and agar dilution susceptibility tests were compared for determining ramoplanin MICs. With a data base of 246 clinical isolates of gram-positive bacteria from 33 U.S. sites, it was shown that (i) agar and tube dilution susceptibility tests gave essentially the same results (93.9% of the te...

AIMS To determine whether quantitative blood culture methods could improve the diagnosis of septicaemic melioidosis. METHODS A comparison of conventional broth based blood cultures, a pour plate method, and a commercial lysis centrifugation (Isolator 10) blood culture system was conducted in 71 Thai patients with severe melioidosis. The time to identification of B pseudomallei was recorded fo...

One hundred methicillin-resistant Staphylococcus aureus (MRSA) strains, isolated between 1983 and 1999, were tested alongside the vancomycin hetero-resistant S. aureus (hVRSA) strain Mu 3, and vancomycin-resistant S. aureus (VRSA) strain Mu 50, for their resistance to vancomycin. This was achieved using the screening method described by Hiramatsu, gradient plates, agar incorporation, standard E...

The alcoholic fermentation of raffinose by yeasts has considerable taxonomic importance. Certain species do not ferment this trisaccharide at all; others ferment only the fructose part; whereas others ferment both parts (fructose and melibiose). All or some strains of a few species ferment melibiose incompletely. Most older methods for testing raffinose fermentation are based on quantitative de...

The most commonly used method for testing the antibiotic susceptibility of aerobic and facultative bacteria is the disk diffusion method. However, some anaerobic bacteria do not grow well enough in anaerobic jars for performance of disk diffusion tests. A modification of the broth-disk method of Schneierson allowed us to determine antibiotic susceptibility in a completely anaerobic environment....