M13 viral strand synthesis is initiated by nicking of the viral strand of the duplex replicative form by the M13 gene II initiator protein at a specific site within a sequence of about 40 base pairs having dyad symmetry. Efficient replication of the M13 viral strand also requires the presence of an adjacent sequence of ca. 100 base pairs. Together these sequences constitute the minimal origin f...

Planview detailed morphological analysis of martian landslides is usually performed using orbital imagery such as from the ConTeXt camera (CTX) at 6 ​m/pix, Colour and Stereo Surface Imaging System (CaSSIS) 4.5 ​m/pix or High-Resolution Science Experiment (HiRISE) 0.25–0.5 ​m/pix. However, topographic information key to fully understand a landslide's formation mechanism its mobility, by estimat...

The replication origins of viral and complementary strands of bacteriophage M13 DNA are contained within a 507-nucleotide intergenic region of the viral genome. Chimeric plasmids have been constructed by inserting restriction endonuclease fragments of the M13 intergenic region into the plasmid pBR322. Replication of these hybrid plasmids, under conditions not permissive for the plasmid replicon...

The growth regulator, PIX (mepiquat chloride l,l-dimethyl-piperdinium chloride), when applied to cotton (Gossypium hirsutum L.) and pecan (Carya illinoensis Koch), caused internode shortening. PIX did not elicit an increase in resistance in cotton to the tobacco budworm[Heliothis virescens (Fab.)], or in pecan to pecan scab (Cladosporium caryigenum (Ell. et Lang) Gottwald]. Also, changes in con...

Physalis macrophysa (PMA) and ixocarpa (PIX) have significant economic, medicinal, ornamental value are often used in the fields of edible fruits, medicinal herbs, plants. In present study, we obtained complete chloroplast (cp) genome sequences PMA PIX, compared to cp 10 species, constructed phylogenetic tree among tribe Physaleae. The results showed that genomes PIX consisted a large single co...

The advent of socio-technical, cyber-physical and artificial intelligence systems has broadened the scope requirements engineering, which must now deal with new classes requirements, concerning ethics, privacy trust. This brings challenges to Requirements Engineering, in particular regarding understanding non-functional behind these types systems. To address this issue, we propose Ontology-base...

Using simple design and selective pressure, we have evolved an artificial M13 bacteriophage coat protein. M13 coat proteins first reside in the bacterial inner membrane and subsequently surround the DNA core of the assembled virus. The artificial coat protein (ACP) was designed and evolved to mimic both functions of the natural M13 coat proteins, but with an inverted orientation. ACP is a non-f...

Superhelical covalently closed circular replicative form DNA (RF I) of coliphage M13 appears as a relaxed molecule that has a base-unpaired region in the form of a bubble (100 to 200 base pairs long) seen in electron micrographs when spread in the presence of formaldehyde and formamide or after pretreatment with glyoxal. S1 endonuclease, specific for single-stranded DNA, converts superhelical M...

Enzymes catalyze organic transformations with exquisite levels of selectivity, including chemoselectivity, stereoselectivity, and substrate selectivity, but the types of reactions catalyzed by enzymes are more limited than those of chemical catalysts. Thus, the convergence of chemical catalysis and biocatalysis can enable enzymatic systems to catalyze abiological reactions with high selectivity...

Adenoviral vectors have been exploited for a wide range of gene therapy applications. Direct genetic modification of the adenovirus capsid proteins has been employed to achieve alteration of vector tropism. We have defined the carboxy-terminus of the minor capsid protein pIX as a locus capable of presenting incorporated ligands on the virus capsid surface. Thus, we sought to exploit the possibi...