BACKGROUND Pasteurella multocida, Mannheimia haemolytica, Histophilus somni and Trueperella pyogenes are four bacterial agents commonly associated with bovine respiratory disease (BRD). In this study a bacterial multiplex real-time PCR (the RespoCheck PCR) was evaluated for the detection in bronchoalveolar lavage fluid (BALF) of these four bacterial agents. RESULTS The analytical sensitivity ...

Mannheimia haemolytica é uma bactéria comensal do trato respiratório de bovinos e associada à infecção desses animais, podendo causar pneumonia, pleurite septicemia. É considerado o principal agente causador doenças respiratórias em bovinos. Relata-se um caso septicemia bezerro causado por M. haemolytica. No exame clínico detectou-se taquicardia, taquipneia, respiração abdominal, hipertermia de...

The respiratory tract contains numerous antimicrobial factors necessary for normal innate pulmonary defense. Although many of these molecules reside in airway surface liquid (ASL) simultaneously, little information exists concerning antagonistic, additive, or synergistic interactions. Since both cationic lysozyme and anionic antimicrobial peptides (AP) are found in high concentrations in ASL, t...

3 National Institute of Public Health, Department of Bacteriology, PO Box 4404 Torshov, 0403 Oslo, Norway The present paper presents the conclusions of a polyphasic investigation of the taxonomy of the t re ha loseneg a t ive [Pasteurella] haemolytica com p I ex. Clusters previously identified by ribotyping and multilocus enzyme electrophoresis (MEE) have been evaluated by 165 rRNA sequencing a...

Several novel 15-membered-ring macrolide agents (azalide 1, triamilides 2 and 3, and the azalide 3,6-ketal 4) were identified as potential antibacterial agents against Mannheimia (formerly named as Pasteurella) haemolytica, Pasteurella multocida, Haemophilus somnus and Actinobacillus pleuropneumoniae, important etiological agents of bovine and porcine respiratory disease. Compound 3 is the majo...

The degree to which the selectin inhibitor TBC1269 reduces neutrophil infiltration in specific microscopic locations of the lung during acute pneumonia of neonates was determined. Neonatal calves were inoculated intrabronchially with Mannheimia (Pasteurella) haemolytica or saline, and lung tissue was collected at 2 and 6 hours postinoculation (PI). One 6-hour group inoculated with M. haemolytic...

In April 2002, an investigation into an outbreak of acute respiratory disease in goats and sheep in Milae (Afar), Ethiopia was conducted. The investigation involved 4 flocks (722 sheep and 750 goats in total) and comprised the disease history, clinical and post-mortem examination, and microbiological analysis of nasal swabs, lung lesions, and pleural fluid samples. Clinically diseased animals e...

Bovine Respiratory Disease is the most costly disease that affects beef and dairy cattle industry. Its etiology is multifactorial, arising from predisposing environmental stress conditions as well as the action of several different respiratory pathogens. This situation has hindered the development of effective control strategies. Although different type of vaccines are available, many currently...

An experimental model of pneumonic pasteurellosis in sheep was used to investigate the role of serum antibody in resistance to this disease. Lambs which had been vaccinated with a sodium salicylate extract of Pasteurella haemolytica type A1 were protected against challenge with PI3 virus followed by P. haemolytica type A1 7 days later. The majority of untreated lambs and lambs which had receive...

BACKGROUND The occurrence of Mannheimia species in healthy sheep has only been investigated to a very limited extend since the genus and its five named species were established. The aim of the present study was to evaluate the occurrence of haemolytic Mannheimia species in apparently healthy sheep originating from four sheep flocks in South-Western Norway. METHODS Typical beta-haemolytic Past...