Background: Protein extracts from chicken egg whites were prepared by utilizing different solvents and we studied the differences in proliferation. Methods: The white extract was using lysate, phosphate-buffered saline (PBS), pure water. vitro mixture experiment carried out to observe effect of preparations on proliferation cells. samples divided into following groups: control group with media,...

Protein aggregation is a hallmark of neurodegenerative disorders such as amyotrophic lateral sclerosis (ALS), Alzheimer's disease (AD), Parkinson's (PD), Huntington's (HD), and so on. To detect analyze soluble or diffuse protein oligomers aggregates, fluorescence correlation spectroscopy (FCS), which can the diffusion speed brightness single particle with molecule sensitivity, has been used. Ho...

Purpose: Whole tumor lysates are promising antigen sources for dendritic cell (DC) therapy as they contain many relevant immunogenic epitopes to help prevent tumor escape. Two common methods of tumor lysate preparations are freeze-thaw processing andUVB irradiation to induce necrosis and apoptosis, respectively. Hypochlorous acid (HOCl) oxidation is a new method for inducing primary necrosis an...

The cutaneous reaction demonstrates that the culture lysate of Streptococcus scarlatinae is approximately ten time more potent in its toxic effect than is the culture filtrate since repeated and carefully controlled human skin tests show that 0.1 cc. of a 1:2000 dilution of lysate reacts equally as well as a similar dose of a 1:250 dilution of culture filtrate (Dick's standard skin unit). Anima...

Background: Cell therapy and cell culture have received much attention in recent decades. Suitable cell growth requires growth supplements such as fetal bovine serum (FBS). FBS is component rich in nutrients, growth factors and supplementary compounds. However, FBS utilization has some limitations including mass production. Therefore, finding alternatives with the same growth promoting effects ...

We have developed and characterized translationally active cell-free systems from Artemia embryos at different developmental times. The optimized lysates from 16 h-developed embryos incorporated radiolabelled amino acids into polypeptides for up to 120 min. The polypeptides synthesized ranged in Mr from 150,000 to 10,000, suggesting that the endogenous mRNA was capable of directing the synthesi...

Naegleria fowleri, a ubiquitous free-living ameba, causes fatal primary amebic meningoencephalitis in humans. N. fowleri trophozoites are known to induce cytopathic changes upon contact with microglial cells, including necrotic and apoptotic cell death and pro-inflammatory cytokine release. In this study, we treated rat microglial cells with amebic lysate to probe contact-independent mechanisms...

MOTIVATION The protein lysate microarray is a developing proteomic technology for measuring protein expression levels in a large number of biological samples simultaneously. A challenge for accurate quantification is the relatively narrow dynamic range associated with the commonly used chromogenic signal detection system. To facilitate accurate measurement of the relative expression levels, eac...

BACKGROUND Monocytes and macrophages contribute to the dysfunction of immune responses in human filariasis. During patent infection monocytes encounter microfilariae in the blood, an event that occurs in asymptomatically infected filariasis patients that are immunologically hyporeactive. AIM To determine whether blood microfilariae directly act on blood monocytes and in vitro generated macrop...