Effects of lyophilization and storage of liver 9,000xg supernatant fraction on the activity of drug metabolizing enzymes in some species of animals were studied. Imipramine metabolizing enzyme activity and the content of cytochrome P-450 were stable for, at least, one month without any loss of the activity when lyophilized liver 9,000xg supernatant fraction of some animal species, i.e., rat, mo...

objective anti-rh d igg is used for the prevention of anti-d antibody production in rh- individuals who have been exposed to rh+ red blood cells. the stability of igg preparations as a solution is low, with a shelf life of a year or more. formulation of anti-rh d igg as a lyophilized preparation would decrease its degradation rate and increases its shelf life. the objective of this study was to...

Ahn, T. H. (University of California, Los Angeles), H. Nishihara, Charles M. Carpenter, and G. V. Taplin. Viability and metabolism of Staphylococcus aureus after freezing, lyophilization, and gamma irradiation. J. Bacteriol. 88:545-552. 1964.-Reproductive viability and oxidative activity on various substrates (alanine, glutamic acid, glucose, lactic acid) were studied in Staphylococcus aureus c...

A major problem of current vaccines is storage stability, often requiring strict maintenance of cold chains. In the course of the eradication of smallpox, a freeze-dried vaccinia virus (Dryvax), which proved to be very stable, was used to overcome this limitation. However, Dryvax needs to be reconstituted before usage and is administered using a bifurcated needle, procedures that pose a number ...

NACHEVA, I., K. ALEKSIEVA, K. DIMOV, D. MITEVA and Tsv. TSVETKOV, 2013. Identification of gamma irradiated lyophilized forest fruits by EPR Spectroscopy. Bulg. J. Agric. Sci., 19: 293-297 The objective of the present study was to prove the radiation treatment of a set of lyophilized forest fruits (blackberry, blueberry, aronia, strawberry and black elder), irradiated with doses of 2 and 4 kGy b...

The present study aims at the phytochemical profiling of lyophilized pure fruit juice powder of Citrus limon and demonstrates its efficacy as an antitumor agent against human breast cancer cell line (MCF-7). The cytotoxicity was evaluated using the changes in cell morphology, cell viability, and DNA fragmentation, the percentage of cell viability was determined by MTT assay. Our results showed ...

Bacillus subtilis 5230 spores were lyophilized in 0.067 M phosphate buffer and stored at 2 to 8 degrees C for 9 to 27 months. The lyophilized spores were reconstituted with buffer or 0.9% saline, and the heat resistance was determined in a thermoresistometer. Lyophilization had no effect on the heat resistance of the spores but did result in a slight decrease in population (</=0.3-logarithm red...

The aim of this study is to investigate the hydration and the dehydration processes of calcium benzoate hydrates (trihydrate and monohydrate), thermotropic mesophases (dehydrated mesophase and lyophilized mesophase) and amorphous state, and the influence of their molecular order on those processes. X-ray analysis revealed that trihydrate has a planar structure composed of two types of planes-on...