Incorporation of (18)O into the CO(2) produced in the bioluminescent oxidation of firefly luciferin was studied. In H(2) (16)O medium with (18)O(2) gas, the product CO(2) contained up to 75% C(16)O(18)O, showing that one O of the product CO(2) arose from the O(2) that oxidized luciferin. This result is consistent with a dioxetane mechanism. Analysis of the mass spectral data of the CO(2) obtain...

The expressions of reference genes used in gene expression studies are assumed to be stable under most circumstances. However, studies had demonstrated that genes assumed to be stably expressed in a species are not necessarily stably expressed in other organisms. This study aims to evaluate the likelihood of genus-specific reference genes for liver using comparable microarray datasets from Sper...

BACKGROUND In cancer cachexia, muscle depletion is related to morbidity and mortality. Muscle-wasting mechanisms in cancer patients are not fully understood. OBJECTIVE We investigated the involvement of the proteolytic systems (proteasome, autophagic-lysosomal, calpain, and caspase) in muscle wasting during cancer cachexia. DESIGN Esophageal cancer patients [n = 14; mean ± SD age: 64.1 ± 6....

Fluorescence of luciferases from Luciola mingrelica (single tryptophan residue, Trp-419) and Photinus pyralis (two tryptophan residues, Trp-417, Trp-426) was studied. Analysis of quenching of tryptophan fluorescence showed that the tryptophan residue conserved in all luciferases is not accessible for charged quenchers, which is explained by the presence of positively and negatively charged amin...

Les Mills BODYPUMPTM is a resistance training group exercise class with low load, high repetition format. Squat in has two key variables: depth and load. The study aim was to determine the effect of these parameters on mean peak EMG amplitude vastus lateralis, gluteus maximus, biceps femoris lateral gastrocnemius. Ten female participants (age 41 ± 9 years, height 161.9 3.8 cm, mass 67.7 7.0 kg)...

ABSTRACT: Andropogon lateralis Nees is a native grass of southern Brazil and one the most frequent specie found in grasslands. The species widely distributed has high degree phenotypic plasticity, which makes it highly adaptable to different edaphoclimatic conditions management. This study aimed evaluate behavior twelve ecotypes A. lateralis, collected regions state Rio Grande do Sul cut three ...

The firefly species Luciola unmunsana was first discovered on the Unmunsan Mountain in Cheongdo-gun, Gyeongsangbuk-do, South Korea and consequently named after mountain. population habitats of this once-abundant have recently decreased significantly due to light environmental pollution caused by industrialization urbanization. This study investigated distribution density L. around ecological la...