Several studies (Eley & Cormier, 1968; Cormier, Eley, Abe & Nakano, 1969; Eley et al. 1970) on intact organisms and partially purified luciferase preparations of Photobacterium phosphoreum (Bergey's Manual of Determinative Bacteriology, I 957) indicated that further studies on the purified luciferase from this bacterium might provide some new understanding about bacterial bioluminescence in gen...

Background. Research in oncology assumes establishment and usage of xenograft animal models, meeting the requirements humanity rationality. Although praised as promising field research, preclinical studies low-molecular-weight inhibitors, antitumor monoclonal antibodies, vaccines, cellular therapy products, CAR-T cells entail continuous control tumor growth dynamics. Luciferase detection biolum...

conclusions according to the high functionality of the produced recombinant vector, it seems a good applicable tool for making an indicator cell line in subsequent basic and drug studies. results recombinant vector, pgl4ltr-luc, has expression levels of more than 50 folds compared to the control when co-transfected with the tax expressing vector into hek 293t cells. moreover, cells display more...

Objective(s): To explore the correlation between expression patterns and functions of miR-383 and LDHA in hepatocellular cancer (HCC). Materials and Methods: We detected the expression of miR-383 and LDHA in 30 HCC tissues and their matched adjacent normal tissues using qRT-PCR. Then we performed MTT assay, foci formation assay, transwell migration assay, glucose uptake assay and lactate produc...

Bioluminescence—i.e., the emission of visible light by living organisms—is defined as a biochemical reaction involving, at least, luciferin substrate, an oxygen derivative, and specialised luciferase enzyme. In some cases, enzyme substrate are durably associated form photoprotein. While this terminology is educatively useful to explain bioluminescence, it gives false idea that all luminous orga...

The thymidine kinase promoter-Renilla luciferase reporter plasmid (pRL-TK) is commonly used as a control for transfection efficiency in the Dual-Luciferase Reporter Assay System. While investigating hormone response elements in the promoters of the androgen-dependent, epididymis-specific EP2 gene, we found that hormone treatment affected the luciferase activity of pRL-TK-transfected cells. In A...