Maris, Nico A., Koenraad F. van der Sluijs, Sandrine Florquin, Alex F. de Vos, Jennie M. Pater, Henk M. Jansen, and Tom van der Poll. Salmeterol, a 2-receptor agonist, attenuates lipopolysaccharide-induced lung inflammation in mice. Am J Physiol Lung Cell Mol Physiol 286: L1122–L1128, 2004. First published January 16, 2004; 10.1152/ajplung.00125.2003.—Lipopolysaccharide is ubiquitously present ...

Maris, Nico A., Koenraad F. van der Sluijs, Sandrine Florquin, Alex F. de Vos, Jennie M. Pater, Henk M. Jansen, and Tom van der Poll. Salmeterol, a 2-receptor agonist, attenuates lipopolysaccharide-induced lung inflammation in mice. Am J Physiol Lung Cell Mol Physiol 286: L1122–L1128, 2004. First published January 16, 2004; 10.1152/ajplung.00125.2003.—Lipopolysaccharide is ubiquitously present ...

BACKGROUND Bronchoconstriction has developed after inhalation of lipopolysaccharide in a dose of 20 micrograms in asthmatic patients and of 200 micrograms in normal subjects. This study set out to determine whether the bronchial response to lipopolysaccharide was related to non-specific bronchial responsiveness and atopy. METHODS Sixteen subjects with a fall in specific airway conductance of ...

The respiratory system is continuously exposed to airborne particles containing lipopolysaccharide. Our laboratory established previously that the hydrophobic surfactant protein C (SP-C) binds to lipopolysaccharide and to one of its cellular receptors, CD14. Here we examined the influence of SP-C, and of a synthetic analog, on some cellular in vitro effects of lipopolysaccharide. When associate...

We investigated the role of soluble guanylate cyclase in lipopolysaccharide-induced hyporesponsiveness to phenylephrine. The effects of phenylephrine on the blood pressure of female Wistar rats were evaluated at 2, 8, and 24 h after lipopolysaccharide injection (12.5 mg/kg i.p.). Vasoconstrictive responses to phenylephrine were reduced 40 to 50% in all time periods. Methylene blue, a soluble gu...

When Escherichia coli Olll:B4 is exposed briefly to ethylenediaminetetraacetate (EDTA) it releases 30 to 50% of its lipopolysaccharide, determined not only by measuring percentage release of colitose, a sugar unique to this polymer, but also by measuring the amount of phenol-extractable lipopolysaccharide remaining with the cell. The fraction released cannot be significantly increased above 50%...

When Escherichia coli Olll:B4 is exposed briefly to ethylenediaminetetraacetate (EDTA) it releases 30 to 50% of its lipopolysaccharide, determined not only by measuring percentage release of colitose, a sugar unique to this polymer, but also by measuring the amount of phenol-extractable lipopolysaccharide remaining with the cell. The fraction released cannot be significantly increased above 50%...

Treatment of gram-negative bacteria with lethal doses of polymyxin B and colistin resulted in the formation of projections of the outer layer of the cell wall. Phages T3, T4, and T7, which use wall lipopolysaccharide as receptors, were specifically prevented from adsorbing to Escherichia coli B cells treated with polymyxin, whereas phages T1, T2, T5, and T6 were not. In the systems of phage P22...

Evidence for transient localization of newly synthesized lipopolysaccharide at the periplasmic face of the inner membrane has been obtained by immunoelectron microscopic techniques. Salmonella typhimurium galE mutants in which O-antigen synthesis is dependent on addition of exogenous galactose were employed, and the distribution and fate of pulse-synthesized O antigen was examined by indirect f...