Purified Cre recombinase protein introduced directly into cultured mammalian cells by lipofection catalyzes both site-specific chromosomal integration of a co-transfected lox targeting vector and precise excision of genomic DNA flanked by directly repeated lox sites. This procedure eliminates the need to transfect cre expression plasmids to activate recombination at lox sites. We used this simp...

background: the multifunctional transforming growth factor beta (tgf-β) is a glycoprotein that exists in three isoforms. tgf-β3 expression increases in fetal wound healing and reduces fibronectin and collagen i and iii deposition, and also improves the architecture of the neodermis which is a combination of blood vessels and connective tissue during wound healing. fibroblasts are key cells in t...

The advent of the CRISPR/Cas9 system has transformed field human genome engineering and created new perspectives in development innovative cell therapies. However, absence a simple, fast efficient delivery method into primary cells been limiting progress CRISPR/Cas9-based Here, we describe an optimized protocol for iTOP-mediated various cells, including T induced pluripotent stem (hiPSCs), Jurk...