The higher order oligomeric state of the Na,K-ATPase alphabeta heterodimer in cell membranes is the subject of controversy. We have utilized the baculovirus-infected insect cell system to express Na,K-ATPase with alpha-subunits bearing either His(6) or FLAG epitopes at the carboxyl terminus. Each of these constructs produced functional Na,K-ATPase alphabeta heterodimers that were delivered to t...

During T cell development in the thymus, pre-T cell receptor (TCR) complexes signal CD4(-) CD8(-) (double negative [DN]) thymocytes to differentiate into CD4(+) CD8(+) (double positive [DP]) thymocytes, and they generate such signals without apparent ligand engagements. Although ligand-independent signaling is unusual and might be unique to the pre-TCR, it is possible that other TCR complexes s...

Let A be a C∗-algebra, and let X be a Banach A-bimodule. B. E. Johnson showed that local derivations from A into X are derivations. We extend this concept of locality to the higher cohomology of a C ∗-algebra and show that, for every n ∈ N, bounded local n-cocycles from A into X are n-cocycles. The study of the local properties of Hochschild cohomology of a Banach algebra was initiated by intro...

The notion of $f$-derivations of UP-algebras is introduced, some useful examples are discussed, and related properties are investigated. Moreover, we show that the fixed set and the kernel of $f$-derivations are UP-subalgebras of UP-algebras,and also give examples to show that the two sets are not UP-ideals of UP-algebras in general.

IFN-alphabeta is a potent immunoregulatory cytokine involved in the defense against viral and bacterial infections. In this study, we describe an as yet undefined IFN-alphabeta-dependent pathway of IFN-gamma induction in mice. This pathway is based on a synergism of IFN-alphabeta and IL-18, and is independent of IL-12 signaling yet dependent on STAT4. In contradiction to current dogma, we show ...

The lack of CD95 in mice is associated with an accumulation of TCR alphabeta+ CD4- CD8- (double-negative (DN)) cells in the lymph nodes (LNs) and other organs. To test the hypothesis that these DN cells arise from TCR alphabeta+ CD8+ cells after activation via the TCR, we have crossed an MHC class I-restricted TCR transgene (tg) onto the lpr genotype to generate two TCR-transgenic experimental ...

In this paper we study the properties of derivations of A B, where A and B are simple separable C*-algebras, and A B is the C*-completion of A B with respect to a C*-norm Yon A B and we will characterize the derivations of A B in terms of the derivations of A and B