A cDNA encoding a chymotrypsinogen-like protein in midguts of the lesser grain borer, Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae) was cloned and sequenced. The 901 bp cDNA contains an 816-nucleotide open reading frame encoding 272-amino acids. The predicted molecular mass and pI of the mature enzyme are 23.7 kDa and 4.64, respectively. The encoded protein includes amino acid sequence m...

Resistance to the fumigant phosphine in stored product insect pests is a global problem. Diagnosis of resistance relies on bioassay developed by FAO that involves mortality assessment after 20-h fumigation pest population at discriminating concentration gas, followed 14-day post assessment. This impractical for monitoring and early detection routine management. We utilized procedure commercial ...

The Tribolium castaneum (Herbst) and Rhyzopertha Dominica (Fab.) are potential pests of stored wheat products that causes destruction to grains all over the world. current investigation was planned examine bifenthrin chlorfenapyr for T. (red flour beetle) R, (Lesser grain borer). A total 50g sample taken in jars. Both were applied at concentrations 0.25, 0.5, 1, 2, 4ppm, separately. Factorial d...

Protein digestion in the lesser grain borer, Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae), results from the action of a complex of serine proteinases present in the midgut. In this study we partially characterized trypsin-like enzyme activity against N-alpha-benzoyl-L-arginine p-nitroanilide (BApNA) in midgut preparations and cloned and sequenced three cDNAs for trypsinogen-like protein...

The lesser grain borer Rhyzopertha dominica (F.) is one of the most destructive insect pests of stored grain. This pest has been controlled successfully by fumigation with phosphine for the last several decades, though strong resistance to phosphine in many countries has raised concern about the long term usefulness of this control method. Previous genetic analysis of strongly resistant (SR) R....

The accuracy of near-infrared spectroscopy (NIRS) for predicting the chronological age of adults of the rice weevil, Sitophilus oryzae (L.); the lesser grain borer, Rhyzopertha dominica (F.); and the red flour beetle, Tribolium castaneum (Herbst), three pests of stored grain, was examined. NIRS-predicted age correlated well with actual age of these three species. Age predictions in S. oryzae by...

Rice weevil (Sitophilus oryzae) and the lesser grain borer (Rhyzopertha dominica) are very important warehouse pests and, therefore, their control is crucial. At a key moment in life of adult Sitophilus spp., obligatory symbiotic nature Sodalis pierantonius bacterium opens up new perspective for natural antibiotics bactericides. In this study, we used nanopore sequencing 16S rRNA barcoding to e...

Tolerant rice accessions were identified from a field study involving 196 at Pandit Jawaharlal of Agriculture and Research Institute, Karaikal for yellow stem borer, Scirpophaga incertulas. in along with the susceptible check (TN1) considered analyzing biochemical parameters viz., chlorophyll content, total sugars, reducing phenols, soluble protein, proline. Higher amounts moderate lesser sugar...

Spinosad and chlorpyrifos-methyl plus deltamethrin efficacy at labeled rates on hard red winter wheat were evaluated against 11 field strains of the red flour beetle, Tribolium castaneum (Herbst); six strains of the sawtoothed grain beetle, Oryzaephilus surinamensis (L.); and two strains of the lesser grain borer, Rhyzopertha dominica (F.), collected mostly from farm-stored grain in Kansas. Adu...