INTRODUCTION The relationships of the biochemical components in seminal plasma and serum, and their origins and physiological effects in male reproductive system have been poorly understood. METHODS Based on the calibration and quality control measures, 26 biochemical markers, in seminal plasma and serum samples from 36 male infertility patients with nonazoospermia were detected and compared....

BACKGROUND Growing evidence indicates that oxidative stress can be a primary cause of male infertility. Non-enzymatic antioxidants play an important protective role against oxidative damages and lipid peroxidation. Human seminal plasma is a natural reservoir of antioxidants. The aim of this study was to determine glutathione (GSH) concentrations, trace element levels (zinc and selenium) and the...

OBJECTIVE This prospective study was designed to investigate the relationship between lipids levels in both serum and seminal plasma and semen parameters. METHODS 631 subfertile men were enrolled. Their obesity-associated markers were measured, and semen parameters were analyzed. Also, seminal plasma and serum TC, TG, HDL and LDL and serum FFA, FSH, LH, total testosterone (TT), estradiol (E2)...

Objective: To investigate the association between malondialdehyde concentration in seminal plasma of infertile men and sperm quality.Methods: This case-control study included 60 male participants ranging from 25-40 years old with half them were fertile other infertile. Semen analysis was performed as per WHO standards, spectrophotometric measurement level done.Results: Results showed that had s...

OBJECTIVE Artificial oocyte activation (AOA) is an effective method to avoid total fertilization failure in human in vitro fertilization-embryo transfer (IVF-ET) cycles. AOA performed using a calcium ionophore can induce calcium oscillation in oocytes and initiate the fertilization process. We evaluated the usefulness of AOA with a calcium ionophore in cases of total fertilization failure in pr...

The aim of the study was to examine the relationships between concentrations of zinc in blood and seminal plasma and sperm quality among infertile and fertile men. One hundred seven male (infertile group) partners of couples who were undergoing investigation for infertility with no known cause for the infertility and 103 men (fertile group) whose wives were pregnant at the time of the study wer...

Infertility is an important aspect of human and animal reproduction and still presents with much etiological ambiguity. As fifty percent of infertility is related to the male partner, molecular investigations on sperm and seminal plasma can lead to new knowledge on male infertility. Several comparisons between fertile and infertile human and other species sperm proteome have shown the existence...

The standard semen analysis is the first line and the most popular laboratory test in the diagnosis of male fertility. It evaluates sperm concentration, motility, morphology and their vitality. However, it is well-known that normal results of semen analysis can not exclude men from the causes of couples' infertility. One of the most important parameters of sperm in its fertilizing potential is ...

Spermatozoa generate reactive oxygen species (ROS) in physiological amounts, which play a role in sperm functions during sperm capacitation, acrosome reaction (AR), and oocyte fusion. In addition, damaged sperm are likely to be the source of ROS. The most important ROS produced by human sperm are hydrogen peroxide, superoxide anion and hydroxyl radicals. Besides, human seminal plasma and sperm ...

BACKGROUND The proteolytic chaperone peptide ubiquitin accumulates in defective human spermatozoa. Immunodetection of ubiquitin in human sperm samples correlates with semen quality and male fertility. METHODS Semen samples from 93 men from couples seeking infertility treatment were separated on a PureSperm density gradient and screened by immunofluorescence microscopy with anti-ubiquitin anti...