Mammary explants from pregnant ewes were cultured in the presence of insulin, cortisol and prolactin, either alone, or in combination. After 2 d of culture, total RNA was extracted from explants and the content of beta-lactoglobulin mRNA was estimated using a specific labelled cDNA probe. The mRNA for beta-lactoglobulin was only deinduced slowly in the absence of hormone during the culture. Pro...

The study was conducted to find the association of -Lactoglobulin locus with the milk performance traits in 157 lactating Sahiwal cows maintained at Cattle yard, NDRI, Karnal. Two different variants of -Lactoglobulin were detected from DNA by PCR-RFLP. It was found that the frequency of B allele was predominantly high (0.84± 0.03) than that of A allele (0.16± 0.03) in the population studied. ...

antigenicity of proteins found in cow's milk is age dependent. this is primarily due to infants possessing a more permeable intestinal wall than that in adults. thus infants may acquire cow's milk allergy during their first year of life. while milk antigen specific ige may cause allergy in susceptible subjects, there is some evidence indicating that milk antigen specific igg may play some role ...

Background When exclusive breastfeeding is not possible, partially hydrolyzed formula (PHF) often used as a starter for infants. Some children develop allergic symptoms, including anaphylaxis, after the first intake of cow protein. Therefore, tolerability PHF in infants with cow's milk allergy (CMA) important information. Partially whey (PHWF) well characterized, but those containing both and c...

86 http://e-aair.org Cow’s milk allergy (CMA) accounts for most diagnosed food allergies, mainly in the first year of life. CMA can cause severe anaphylaxis. CMA management is based on strict avoidance and the prescription of rescue medication. Cow’s milk (CM) contains more than 40 proteins, including casein (Bos d 8), -lactalbumin (Bos d 4), and -lactoglobulin (Bos d 5), identified as major mi...

High expression and secretion of recombinant ovine beta-lactoglobulin has been achieved in the yeast Kluyveromyces lactis. The yield of beta-lactoglobulin is 40-50 mg per litre of culture supernatant and accounts for approx. 72% of the total secreted protein. Constitutive expression is under the control of the Saccharomyces cerevisiae phosphoglycerate kinase promoter from an intronless version ...

I3C-NMR studies of modified 6-lactoglobulins, cyanylated at the single thiol group, have provided evidence to support the location of the thiol at Cys-121 [ I J and useful information about the nuclear relaxation behaviour of the thiocyanate carbons of cyanylated thiol groups in proteins [2]. The O-lactoglobulin monomer contains two tryptophan residues. Trp-19 is buried deep inside the hydropho...

The simultaneous separation of bovine whey proteins [alpha-lactalbumin and beta-lactoglobulin (A+B)] and soybean proteins was performed, for the first time, by capillary electrophoresis. Different experimental conditions were tested. The most suitable consisted of 0.050 M phosphate buffer (pH 8) with 1 M urea and 1.2 mg/ml methylhydroxyethylcellulose, UV detection at 280 nm, 15 kV applied volta...

The number of studies regarding the influence of genetic and environmental factors of ovine milk properties of non-dairy breeds is limited. The aim of this study was to determine the influence of breed, -lactoglobulin variants and somatic cell count on the content of the principal milk ingredients and whey protein components. The study was carried out on 60 Polish Lowland and Polish Heath ewes...