Artisanal cheeses are known as the source of beneficial lactic acid bacteria (LAB). Therefore, this study aimed to isolate and characterize LAB with different proteolytic activities from Iranian artisanal white cheeses. The isolates were classified into low, medium, high activity clusters via K-means clustering identified Lactiplantibacillus (Lpb.) pentosus L11, Lpb. plantarum L33, Enterococcus...

Guanosine diphosphate esterified at the beta-phosphate group with the photolabile 4-azidophenol [1-(4-azidophenyl)-2-(5'-guanyl)pyrophosphate] was found to inhibit GDP binding to the ribosomes of E. coli. UV-irradiation of the fusidic acid-stabilized complex among ribosomes, elongation factor G, and the azidophenyl-GDP, results in selective attachment of the photo-affinity label to the proteins...

K+-depleted 60S ribosomal subunits from rat liver were submitted to a mild treatment with ribonuclease T1. Ribonucleoprotein fragments could be separated on sucrose gradients only when the digested subunits were partially deproteinized with a high KCl concentration (0.6 M) which removed seven proteins more or less completely and 5S RNA. The RNA and protein content of each fragment has been char...

Institute of Molecular Biology proteins dimerize through their N-terminal domains, proCopenhagen University ducing a “stalk” structure on the 50S subunit with a Sølvgade 83 flexible hinge region that probably allows the C-terminal DK1307-Copenhagen K domains of the dimers to move relative to one another Denmark and to the ribosomal surface (Traut et al., 1995). The rRNA region is also involved,...