Biofilm formation by Pseudomonas aeruginosa has been implicated in the pathology of chronic wounds. Both the d and l isoforms of tryptophan inhibited P. aeruginosa biofilm formation on tissue culture plates, with an equimolar ratio of d and l isoforms producing the greatest inhibitory effect. Addition of d-/l-tryptophan to existing biofilms inhibited further biofilm growth and caused partial bi...

L-Tryptophan-2,3-dioxygenases (EC 1.13.1.12), purified to homogeneity from Pseudomonas acidovorans and from rat liver, have been shown to contain as bound cofactors, 2 g atoms copper and 2 moles of heme per mole of tetrameric enzyme. Kinetic and spectral studies, utilizing the metal complexing agents diethyldithiocarbamate (Cu2+ specific), bathocuproinesulfonate (Cu+ specific), and bathophenant...

Abstract Rauwolfia tetraphylla L., is an important medicinal plant in Apocynaceae family and recognized as alternative source to serpentina terms of anti-hypertensive alkaloid production i.e. reserpine. In view this, the present study conducted estimate reserpine content different parts (leaf, stem root) field grown plants (2 years old), tissue cultured plantlets (R 1 ) (two months old) cell su...

Two feeding trials with baby pigs were conducted to evaluate the effects of additions of L-tryptophan and L-giutamic acid to a low protein (16%) starter diet on performance and plasma urea nitrogen, a-amino nitrogen and free tryptophan. In experiment 1, but not in experiment 2, glutamic acid addition (3.36%) improved rate of gain and feed efficiency. Tryptophan addition did not improve performa...

Acetate accumulation during the fermentation process of Escherichia coli FB-04, an L-tryptophan production strain, is detrimental to L-tryptophan production. In an initial attempt to reduce acetate formation, the phosphate acetyltransferase gene (pta) from E. coli FB-04 was deleted, forming strain FB-04(Δpta). Unfortunately, FB-04(Δpta) exhibited a growth defect. Therefore, pta was replaced wit...

The title compound, C(11)H(12)N(2)O(2)·3C(6)H(5)NO(3), comprises a zwitterionic amino acid formed by two nearly planar groups: (i) the indole ring and Cβ, and (ii) the carboxyl group, Cα, as well as the amine N atom, with r.m.s. deviations of 0.0084 and 0.0038 Å, respectively. The angle between these idealized planes is 39.47 (9)°. The amine group of the amino acid is in a syn (-sc) arrangement...

The redetermined crystal structure of the title compound, C(11)H(13)N(2)O(2) (+)·Br(-), is reported. Data collection at 100 K about three crystallographic axes resulted in a crystal structure with significantly higher precision in comparison to the two-dimensional data collected at 176 K [Takigawa et al. [(1966) Bull. Chem. Soc. Jpn, 39, 2369-2378]. The carboxyl group and indole ring system are...

1. Equimolar amounts of gamma-L-glutamyl-L-3,4-dihydroxyphenylalanine (gludopa) and gamma-L-glutamyl-5-hydroxy-L-tryptophan were infused separately and together in eight healthy, salt-replete male subjects in a placebo-controlled, cross-over study to investigate whether the administration of one amine precursor affects the renal metabolism of the other and to determine whether dopamine or 5-hyd...

twenty six rhizobium strains were isolated from root nodules of sesbania sesban (l.) merr. collected from different regions of andhra pradesh. all the 26 rhizobium strains produced indole acetic acid (iaa), but maximum amount was produced by only five strains in yeast extract mannitol  (yem) medium supplemented with l-tryptophan. the strains were found to elaborate maximum iaa when fed with 2.5...