We report the atomic-resolution (1.3 Å) X-ray crystal structure of an open conformation dapE-encoded N-succinyl-l,l-diaminopimelic acid desuccinylase (DapE, EC 3.5.1.18) from Neisseria meningitidis. This [Protein Data Bank (PDB) entry 5UEJ] contains two bound sulfate ions in active site that mimic binding terminal carboxylates (l,l-SDAP) substrate. demonstrated inhibition DapE by (IC50 = 13.8 ±...

background: fish have been at high risk of atrazine toxicity. comparative atrazine toxicity on the tissues of clarias gariepinus is scanty. therefore, acute and chronic effects of atrazine on some biochemical parameters in clarias gariepinus were investigated in this study. methods: atrazine toxicity was determined by assessing the responses of glucose, protein, alanine aminotransferase (alt), ...

background: heat shock protein 70 (hsp70) is present in all organisms studied so far, and is a major immunogen in infections caused by pathogens including leishmania spp. objective: the aim of this study was to clone and express hsp70 from l. infantum strain mcan/ir/96/lon-49 and evaluate antibody response against hsp70 in visceral leishmaniasis (vl). methods: the l. infantum hsp70 gene segment...

Recombinant protein production in Pichia pastoris is based on alcohol oxidase promoterswhich are regulated by methanol. However, the use of methanol has several disadvantages,which is why current trends in bioprocess development with Pichia pastoris (P. pastoris) arefocusing on methanol mixed feeding strategies. This work aimed to develop a new experimentalmethod and compare the effect of vario...

Background: Pekmez is an important fruit-based food of Turkish culinary culture. The aim of this study is to determine the levels of acrylamide (AA), 5-hydroxymethyl-2-furfural (HMF) and other selected parameters in grape, mulberry and carob Pekmez. Methods: AA and HMF were analyzed by Liquid Chromatography and High Performance Liquid Chromatography, respectively. Also, glucose, fructose, pH, ...

In this study, Glucoamylase production by Aspergillus Niger was investigated under solid state conditions with low cost by-products of agricultural processes as substrate. Highest enzyme production was observed when a combination of wheat bran (WB) and corn flour (CF) was used as compared to WB+ rice bran, WB+ rice flour and WB alone. Different additions of (CF) were tested and WB+ 10% CF showe...

 The a im of th is paper was to study the effects w hey pro t e in c oat i ng on ch e m ic al a nd physic al properties of gut t ed K i l ka dur i ng f roz en stor a ge. Coating of Kilka has done by dipping in whey protein solution with different concentrations of 3, 7, 10 and 13%, for 1h. Then, after being packed in polyethylene dishes, they were covered in cellophane blanket and stored in -18...

The aim of investigation was to determine the effects of aspect (north and south) on some morphological [plant height (cm), number of stems per plant, main stem diameter (mm), number of leaves per main stem, petiole length (cm), leaflet length (cm), leaflet width (cm), leaf: stem ratio] and chemical [crude protein (%), crude cellulose (%), calcium (%), phosphorus (%), potassium (%) and magnesiu...

Recombinant protein production in Pichia pastoris is based on alcohol oxidase promoterswhich are regulated by methanol. However, the use of methanol has several disadvantages,which is why current trends in bioprocess development with Pichia pastoris (P. pastoris) arefocusing on methanol mixed feeding strategies. This work aimed to develop a new experimentalmethod and compare the effect of vario...