Pseudomonas butanovora possesses an alcohol-inducible alkane monooxygenase, butane monooxygenase (BMO), that initiates growth on C(2)-C(9) alkanes. A lacZ transcriptional reporter strain, P. butanovora bmoX::lacZ, in which the BMO promoter controls the expression of beta-galactosidase activity, was used to show that 1-butanol induced the BMO promoter in the presence or absence of O(2) when lact...

SUMMARY The deoxyribonudeotide, total nucleotide, and protein con tent of single mononuclear cells derived directly from the peripheral blood buffy coats of patients with infectious mono nucleosis were determined cytochemically by means of ultra microspectrophotometry and ultramicrointerferometry. A small fraction of cells in the populations derived from each patient was found to be in the 5-or...

To investigate the role of AP-1 transcription factors in mediating retinoid-induced growth suppression of breast cells, we studied the sensi tivity of MCF7 breast cancer cells with different levels of AP-1 activity to all-frans retinole acid (atRA). AP-1 activity was increased in MCF7 cells by stably transfecting c-jun cDNA into these cells. Parental and vectortransfected MCF7 cells, which were...

The effect of prolonged UV irradiation (mostly 2537 A) on the catalase activity of an aqueous yeast suspension was divisible into 4 periods. First, the period during which the cells lost their ability to form colonies, but during which no change in catalase activity was noted. Second, the period during which a considerable rise in catalase activity (Euler effect) occurred. The Euler effect was ...

BACKGROUND & OBJECTIVES Plasmodium falciparum is the leading cause of mortality and causes cerebral malaria associated with sequestration caused by cytoadherence of the trophozoite and schizont-infected erythrocytes to the endothelial cells of the deep vascular beds in the brain. Pathophysiology of malaria is complicated by rosetting. Rosetting is a process of binding of uninfected erythrocytes...

Following a recent demonstration that H,K-ATPase can active transport Na+ at a low rate (Polvani, C., Sachs, G., and Blostein, R. (1989) J. Biol. Chem. 264, 17854-17859), we have looked for and found effects of Na+ ions on the conformational state of gastric H,K-ATPase labeled with fluorescein isothiocyanate. Na+ ions reverse the K(+)-induced quench of the fluorescein fluorescence and somewhat ...

Programmed cell death plays an important role during thymocyte development, since a vast majority (97%) of mouse cortical thymocytes die in thymus, whereas only 3% of these cells are rescued from cell death and positively selected. Although it seems well established that thymocyte fate depends upon appropriate surface-expressed T cell receptor, little is known about the molecular mechanism(s) r...

A Poptosis, the process by which cells commit suicide, is ubiquitous in both normal and diseased tissues, and its mechanism is conserved in animal species from humans at least to nematodes. Apoptotic cells undergo a stereotypical set of changes--shrinkage, cleavage of nuclear DNA, condensation of chromatin, fragmentation of the cytoplasm-that distinguish them from cells dying by other means. In...