The architecture of cellular RNA polymerases (RNAPs) dictates that transcription can begin only after promoter DNA bends into a deep channel and the start site nucleotide (+1) binds in the active site located on the channel floor. Formation of this transcriptionally competent "open" complex (RP(o)) by Escherichia coli RNAP at the lambdaP(R) promoter is greatly accelerated by DNA upstream of bas...

The chemical probes potassium permanganate (KMnO4) and diethylpyrocarbonate (DEPC) can be used to study the conformational flexibility of short tracts of adenine (A-tracts) present in DNA. With these probes, we demonstrate that a novel distortion is induced in a 5 base pair A-tract at low temperature. Formation of this distorted A-tract structure, which occurs in a DNA fragment from the promote...

Here we present an in vivo footprinting analysis of the Saccharomyces cerevisiae HSP82 promoter. Consistent with current models, we find that yeast heat shock factor (HSF) binds to strong heat shock elements (HSEs) in non-heat-shocked cells. Upon heat shock, however, additional binding of HSF becomes apparent at weak HSEs of the promoter as well. Recovery from heat shock results in a dramatic r...

Four pharmaceuticals (ranitidine, nizatidine, doxylamine, and carbinoxamine) were selected as model compounds to assess the efficiency of four oxidants (ozone (O3), chlorine (Cl2), chlorine dioxide (ClO2) and potassium permanganate (KMnO4)) on the removal of amine-based pharmaceutical and personal care products (PPCPs), as well as the reduction of their N-Nitrosodimethylamine formation potentia...

Vegetables generally contain more or less oxalic acid, which is produced by metabolism in cytoplasm.12 Most of the oxalic acid is fixed as calcium oxalate to protect protoplasm from its toxic action.3 Vegetables containing a considerable quantity of oxalic acid are usually boiled before being cooked to remove any harshness.4 The oxalic acid contained in food is determined by the KMnO4 titration...

This article discloses the synthesis and characterization of novel benzothiazine derivatives obtained via condensation followed by cyclization of substituted 2-aminobenzenethiols with oxalic acid and ethyl malonate respectively. Their sulfone derivatives were prepared via reacting them with KMnO4 in glacial acetic acid. The structure of the compounds were confirmed by spectral studies (IR, H NM...

Introduction: Potassium Permanganate (KMnO4) is an uncommon cause of caustic ingestion in teenagers and adults; only case reports are found the recent literature. At Ngwelezana Hospital South Africa, KMnO4 not indication for admission. readily available as used most households recommended medicinal purposes by traditional health practitioners. Aim: To ascertain reasons ingestion, extent severit...

Bitter gourd is a widely consumed cucurbitaceous vegetable rich in several nutrients and phytochemicals. The fruit also used traditional medicine systems to cure variety of ailments. However, bitter has very short postharvest life 3-4 days at ambient conditions, due which it suffers significant loss after harvest. In the present study, with aim extend storage life, fruits were packed corrugated...