BACKGROUND Several different commercial one-cycle labeling kits are available for preparation of the target for use with the Affymetrix GeneChip platform. However, there have been no evaluations of these different kits to determine if comparable results were generated. We report on the cRNA target synthesis, labeling efficiency and hybridization results using the One-Cycle Target Labeling Assay...

Radionuclide Tc99m DTPA (Diethylenetriaminepenta Acetic Acid) renal imaging is being used to assist in the evaluation of overall GFR, the intra-renal transit time, the cortical transit time, response to frusimide injection, diagnosis of ATN and rejection in renal transplant cases. A study was conducted at PINSTECH in order to prepare DTPA kit locally and evaluate the labeling efficiency as well...

Patients with GISTs most commonly have KIT and PDGFRA mutations. The therapeutic agents are determined based on these sites of mutation. Imatinib and sunitinib are proven to be effective against GIST, and these agents are now the standard treatment options for GISTs. Moreover, several novel molecular-targeted agents are under development, particularly regorafenib and nilotinib which have phase ...

To prevent food-borne bacterial diseases and to trace bacterial contamination events to foods, microbial source tracking (MST) methods provide important epidemiological information. To apply molecular methods to MST, it is necessary not only to amplify bacterial cells to detection limit levels but also to prepare DNA with reduced inhibitory compounds and contamination. Isolates carrying the Clo...

STR typing in forensic genetics has been performed traditionally using capillary electrophoresis (CE). However, CE-based method has some limitations: a small number of STR loci can be used; stutter products, dye artifacts and low level alleles. Massively parallel sequencing (MPS) has been considered a viable technology in recent years allowing high-throughput coverage at a relatively affordable...

BACKGROUND Automated sample preparation systems must meet the demands of routine diagnostics laboratories with regard to performance characteristics and compatibility with downstream assays. OBJECTIVES In this study, the performance of QIAGEN EZ1 DSP Virus Kit on the BioRobot EZ1 DSP was evaluated in combination with the Abbott RealTime HIV-1, HCV, and HBV assays, followed by thermalcycling a...

Here, we describe our investigation of the preparation and use of chemically labeled DNA probes for fluorescence in situ hybridization (FISH) experiments. Compared to enzymatic labeling of DNA, the chemical labeling process is expected to be significantly more robust, cheaper and easier to scale up (1,2,8). Running a major probe isolation/preparation facility in the western US, we have been pro...