A kinetic priority queue is a kinetic data structure which determines the largest element in a collection of continuously changing numbers subject to insertions and deletions. Due to its importance, many different constructions have been suggested in the literature, each with its pros and cons. We propose a simple construction that takes advantage of randomization to achieve optimal locality an...

Activity and kinetics of arylsulfatase A (ASA, EC 3.1.6.8) were analyzed in leukocyte homogenates derived from patients suffering from cerebral palsy. Lower ASA activity was found in the patients' leukocytes than in controls, as determined by spectrophotometry using chromogenic substrate p-nitrocatechol sulfate (p-NCS). Kinetic parameters, K(m) and v(max), for leukocyte ASA were determined from...

The kinetics of the reactions between [Ni(MeOH)6](2+) (hereafter Ni(2+)) and a variety of neutral Schiff base multidentate ligands have been measured in methanol at 25.0 °C using stopped-flow spectrophotometry. The ligands contain a variety of different potential donor sites (phenolic OH, imine N, pyridyl N and NH groups), different structural components and substituents. The kinetic studies ex...

Azo dyes are produced in very large quantities and used as reactive dyes by the cotton industry. They are very stable and resistant to chemical processes. Therefore, their removal from industrial effluents by conventional methods (mechanical screening, sedimentation, biological treatment, biofilters) alone is usually not very effective. This problem can be solved by combining the conventional t...

A kinetic and spectroscopic characterization of the ferryl intermediate (APO-II) from APO, the heme-thiolate peroxygenase from Agrocybe aegerita, is described. APO-II was generated by reaction of the ferric enzyme with metachloroperoxybenzoic acid in the presence of nitroxyl radicals and detected with the use of rapid-mixing stopped-flow UV-visible (UV-vis) spectroscopy. The nitroxyl radicals s...

Photoreactions of the fourth rhodopsin-like pigment, phoborhodopsin (pR480), of Hulobacrerium halobium were studied by low-temperature spectrophotometry. Upon irradiation of pR480 at -80 as well as 170°C with 436 nm light, a new intermediate having a difference absorption maximum at about 520 nm was produced. We designate this intermediate ‘P520’. Prolonged irradiation caused the formation of a...

Rapid, precise, accurate, and specific ratiospectra derivative spectrophotometry and a simple UV spectrophotometry using simultaneous equation methods are developed for the simultaneous determination of bisoprolol fumarate and hydrochlorothiazide from combined pharmaceutical dosage forms. For ratiospectra derivative spectrophotometry, the amplitudes were measured at 202.6 nm for bisoprolol fuma...

In the standard nanosecond laser photolysis method for kinetic studies, a Q-switched laser generates transient species, and absorption spectrophotometry provides a measure of their concentrations. The sample is placed between the monitoring source (a pulsed xenon arc or a flash lamp) and a monochromator, and a photomultiplier tube (PMT) is used for measuring the intensity of the light leaving t...