1. A quantitative turbidimetric method for the estimation of hyaluronidase activity, based on the ability of the enzyme to decrease the capacity of the polysaccharide to precipitate acidified protein has been developed. Two units of hyaluronidase, by this method, are equivalent to one viscosity-reducing unit. 2. Hyaluronidase added to a phagocytic system containing defibrinated human blood, imm...

A simple and rapid turbidimetric method for the assay of hyaluronidase (1) has proved of great aid in the purification of this enzyme from bovine testes (2). Chondroitinsulfuric acid (CSA) is related chemically and biologically to hyaluronic acid, the substrate of hyaluronidase. The presence of a previously identified (3) CSA-depolymerizing activity (chondroitinase) in crude testicular preparat...

It is well known that some XML elements correspond to objects (in the sense of object-orientation) and others do not. The question we consider in this paper is what benefits we can derive from paying attention to such object semantics, particularly for the problem of keyword queries. Keyword queries against XML data have been studied extensively in recent years, with several lowest-common-ances...

BACKGROUND Salivary hyaluronidases have been described in a few bloodsucking arthropods. However, very little is known about the presence of this enzyme in various bloodsucking insects and no data are available on its effect on transmitted microorganisms. Here, we studied hyaluronidase activity in thirteen bloodsucking insects belonging to four different orders. In addition, we assessed the eff...

Seventy-four cultures of Pasteurella multocida representing all four capsular types, A, B, D, and C, from various animal species and diseases were examined for the production of hyaluronidase by two procedures. In one, hyaluronidase production was determined by the depolymerization of streptococcal capsular hyaluronic acid, and in the other, production was determined by degradation of sodium hy...

A comparative study of spreading factor and hyaluronidase in preparations from various sources revealed the following points of similarity and dissimilarity in the two reactions. 1. Similarities: (a) All preparations containing hyaluronidase also produced spreading. (b) Heating at 65 degrees and 100 degrees C. for 30 minutes produced a comparable effect on both reactions. (c) The demonstration ...

OBJECTIVE Functional studies show that disruption of endothelial surface layer (ESL) is accompanied by enhanced sensitivity of the vasculature towards atherogenic stimuli. However, relevance of ESL disruption as causal mechanism for vascular dysfunction remains to be demonstrated. We examined if loss of ESL through enzymatic degradation would affect vascular barrier properties in an atherogenic...

Embryonic chick chondrocytes were cultured in the presence or absence of different preparations of testicular hyaluronidase. This treatment inhibited the accumulation of cartilage matrix as indicated by phase-contrast microscopy, by Alcian green staining, and by accumulation of 35S-labeled material. In addition, most preparations of testicular hyaluronidase caused a conversion of the cells to a...

One hundred and seven (41%) of 262 isolates of Streptococcus milleri, from human sources, produced hyaluronidase. Hyaluronidase production was commoner in beta haemolytic isolates 32 of 39 (82%), many of which were of Lancefield group F. But hyaluronidase was also found in alpha and non-haemolytic isolates, and in groups A, C, G, and non-groupable isolates. There was a strong association betwee...