The oxidation of acetic acid in the dark by the non-sulfur purple bacterium, Rhodospirillum rubrum, was shown by van Niel (1, 2) to require the presence of either carbon dioxide or trace amounts of the Cb-dicarboxylic acids of the Krebs cycle. These results could readily be interpreted to mean that the oxidation of acetic acid proceeds by way of the Krebs cycle, the presence of COZ being requir...

In a previous publication (1) from this laboratory, it was demonstrated that the transamination of amino acids to a-ketoglutaric acid is a reaction of wide scope. Although the data reported in the earlier paper permitted the computation of Q transaminase values, these values were of limited quantitative significance since no attempt was made to study the kinetics of these reaction systems, the ...

ISOCITRIC DEHYDROGENASE (lCD)5 is one of the newer enzymes that has recently come to the attention of clinical medicine. Sterkel et at. (1) have shown that serum lCD is elevated in hepatitis and to a lesser extent in some malignancies. Bowers and McDuffee (2) observed marked elevations of serum lCD in 2 patients who at autopsy showed extensive central lobular liver cell necrosis. The enzyme act...

A new method to assay the enzyme, leucine aminopeptidase (LAP; L-leucyl-peptide hydrolase.-3.4.1.1), is described. The technic is an extension of the conventional glutamic-pyruvic transaminase (GPT) reaction. Serum LAP is allowed to react with the substrate L-leucyl-L-alanine to quantitatively generate alanine. This alanine, in the presence of excess -ketoglutaric acid and GPT, rapidly forms py...

A resting cell system was developed for bacteriocin Lac-B23 production from Lactobacillus paracasei J23. The resting cell medium contained (g/L): Glucose 20, Sodium acetate 5.0, MnSO4 0.25 MgSO4 0.5, Ammoniumhydrogencitrate 1.0, KH2PO4 1.0. The resting cell incubation time and temperature were 20 h and 37 °C and the effects of exogenous factors, including amino acids, glycerol, pyruvic acid, an...

The type and content of organic acids in fruits are important factors that determine the traits, such as nutrition flavor fruits. Fruit weight size also very economic characters fruit breeding research. To investigate inheritance patterns longan, acid contents, weight, were measured distant hybridization progenies longan ‘Huanongzao’ lychee ‘Ziniangxi’. results revealed malic accumulation exhib...