Trimetazidine is a clinically effective antianginal agent that has no negative inotropic or vasodilator properties. Although it is thought to have direct cytoprotective actions on the myocardium, the mechanism(s) by which this occurs is as yet undefined. In this study, we determined what effects trimetazidine has on both fatty acid and glucose metabolism in isolated working rat hearts and on th...

This over-all equation is thought to consist of the repetition of a series of reactions, each series being initiated by the condensation of malonyl coenzyme A with an unsubstituted straight chain acyl compound to form ,&ketoacyl-S-enzyme (5, 8). A series is then presumably concluded by reactions in which the P-ketoacyl compound is reduced and dehydrated to form an acyl compound 2 carbon atoms l...

the biosynthetic pathways of saturated and unsaturated fatty acids consist of many steps controlled by various enzymes. one of the methods for improving oil quality is to change the fatty acid profile through genetic manipulation which requires isolation and characterization of the genes and other cis-acting elements, such as the promoter, involved in fatty acid biosynthesis. b-ketoacyl-coa syn...

Fatty acids play many roles in plants, but the function of some key genes involved fatty acid biosynthesis plant development are not yet properly understood. Here, we clone two β-ketoacyl-[ACP] reductase (KAR) from sunflower, HaKAR1 and HaKAR2, characterize their functional roles. The enzymes cloned were only copies present sunflower genome. Both displayed a high degree similarity, promoters in...

Enzymic activities and gene expression of oleoyl-CoA elongase were studied during seed development using two different rapeseed cultivars, high-erucic-acid rapeseed (HEAR) and low-erucic-acid rapeseed (LEAR). The overall elongase activities were maximal in HEAR between the fourth and eighth weeks after pollination (WAP) and absent in LEAR. The 3-ketoacyl-CoA synthase (condensing enzyme, CE) mRN...

Acyl carrier proteins (ACPs) are central hubs in polyketide and fatty acid biosynthetic pathways, but the fast motions of the ACP's phosphopantetheine (Ppant) arm make its conformational dynamics difficult to capture using traditional spectroscopic approaches. Here we report that converting the terminal thiol of Escherichia coli ACP's Ppant arm into a thiocyanate activates this site to form a s...

BACKGROUND There is an urgent need for the discovery and development of new drugs against Mycobacterium tuberculosis, the causative agent of tuberculosis, especially due to the recent emergence of multi-drug and extensively-drug resistant strains. Herein, we have examined the susceptibility of mycobacteria to the natural product platensimycin. METHODS AND FINDINGS We have demonstrated that pl...