Program Number: 1300 Presentation Time: 8:30 AM–8:45 AM Retinoic Acid Improves Keratocyte Phenotype in a 3D Model of the Corneal Stroma Ricardo M. Gouveia, Fadhilah Z. Abidin, Che J. Connon. Institute of Genetic Medicine, Newcastle University, Newcastle-upon-Tyne, United Kingdom. Purpose: Retinoic Acid (RA) is a metabolite of vitamin A fundamental for the correct function and development of the...

The interface dynamics of a 3D cell immersed in extracellular matrix is investigated. We suggest generalization known 2D minimal phase field model suggested Ziebert et al. [ J. R. Soc. Interface 9 (2012) 1084–1092] for the description keratocyte motility. Our consists two coupled evolution equations order parameter and three-dimensional vector describing actin network polarization (orientation)...

PURPOSE To demonstrate whether the original keratocyte phenotype is maintained with proliferative activity by suppressing TGF-beta signaling in rhesus monkey keratocytes expanded in a serum-free and low-[Ca2+] medium. METHODS Rhesus monkey keratocytes were isolated from central corneal buttons by collagenase digestion for 16 hours, seeded on plastic in Dulbecco's modified Eagle's medium (DMEM...

It remains elusive as to what bone marrow (BM) cell types infiltrate into injured and/or diseased tissues and subsequently differentiate to assume the phenotype of residential cells, for example, neurons, cardiac myocytes, keratocytes, etc., to repair damaged tissue. Here, we examined the possibility of whether BM cell invasion via circulation into uninjured and injured corneas could assume a k...

Keratocytes, the resident cells of the corneal stroma, are responsible for maintaining turnover of this tissue by synthesizing extracellular matrix components. When the cornea is injured, the keratocytes migrate to the wounded site and participate in the stromal wound healing. The neuropeptide substance P (SP), which is also known to be produced by non-neuronal cells, has previously been implic...

PURPOSE To isolate multipotent precursors from the rabbit corneal stroma and to compare the distribution and proliferative capacity of keratocyte precursors obtained from the central and peripheral regions of the corneal stroma. METHODS The rabbit corneal stroma was divided into a peripheral region (6.0-10.0 mm in diameter) and a central region (6.0 mm in diameter). A sphere-forming assay was...

BACKGROUND Murine models of high-risk and low-risk corneal transplantation were used to determine the role of keratocyte apoptosis in the failure of orthotopic allogeneic corneal transplants. MATERIALS AND METHODS Normal (low-risk, low-rejecting) and inflamed/vascularized (high-risk, high-rejecting) BALB/c recipient beds received fully mismatched C57BL/6 corneal allografts. Apoptosis was dete...