The keratinase (kerA) gene from Bacillus licheniformis PWD-1 was expressed and purified in insect cells. First, the sequence encoding Ker-His-Flag was designed based on the amino acid sequence of the protein and peptide and codon optimization in order to ensure the high expression in insect cells. In the next step, the synthetic DNA was inserted into the pUC57 vector and then sub-cloned in the ...

Keratinase producing microorganisms are being increasingly utilized for degradation and recycling of poultry feather waste. Two native strains BF11 (Bacillus subtilis) and BF21 (Bacillus cereus) degrading keratin completely were characterized. The native strains produced more than 10 KU/mL of enzyme. Strain improvement resulted in isolation of MBF11 and MBF21 from BF11 and BF21 isolates, respec...

Keratins are tough, fibrous proteins found as the major protein component in hair. Chemical hair removal is harsh and results severe toxicity pollution, hence, a need for non-chemical enzymatic process. The aim of this study to characterise keratinase from isolated keratin-degrading Bacillus licheniformis-K51, subtilis-K50 sp.-K53 investigate its potential removal. collagenolytic ability kerati...

The keratinolytic activities of dermatophytes which parasitize skin, nails, and hair have been described previously (1, 3, 6, 8); however, the isolation of a keratinase from these organisms has not yet been reported. Trichophyton mentagrophytes var. granulosum (T. granulosum Sabouraud, 1909) was grown in a keratin medium composed of (per liter): horse hair, 2.5 g; glucose, 0.9 g; MgSO4.7H20, 0....

Azure dye-impregnated sheep's wool keratin (keratin azure) was incorporated in a high pH medium and overlaid on a keratin-free basal medium. The release and diffusion of the azure dye into the lower layer indicated production of keratinase. Fifty-eight fungal taxa, including 49 members of the Arthrodermataceae, Gymnoascaceae and Onygenaceae (Order Onygenales), were assessed for keratin degradat...

Abstract Incubation parameters used for the creation of a protein lysate from enzymatically degraded waste feathers using crude keratinase produced by Laceyella sacchari strain YNDH were optimized Response Surface Methodology (RSM); amino acids quantification was also estimated. The optimization elevated total to 2089.5 µg/ml through application following optimal conditions: time 20.2 h, feathe...