In this study, the solubilization of waste coarse wool as a precursory step for large-scale valorization keratin was investigated using green deep eutectic solvent (DES) based on L-cysteine and lactic acid. The investigation undertaken via response surface methodology Box–Behnken design four process variables temperature (70–110 °C), dissolution time (2–10 h), mass (0.5–2.5 g) in 20 mL acid, lo...

Chemical contaminants or their metabolites may bind to and react with keratin proteins in the stratum corneum of the skin. Here, we present a tape-stripping method for the removal and quantification of keratin from the stratum corneum for normalization of extracted concentrations of naphthalene (as a marker for jet fuel exposure) from 12 human volunteers before and after exposure to jet fuel (J...

In our research, we explored the relationship between Keratin 26 and the regulation of fine hair, BMP signaling pathway, MT, FGF5, and IGF-I. The result of hybridization in situ revealed that Keratin 26 was specially expressed in cortex of skin hair follicles; the result of immunohistochemistry indicated that Keratin 26 was expressed in internal root sheath, external root sheath. Then, Real-tim...

Chicken feathers are valuable solid waste that mainly contains insoluble proteins called keratin. Naturally, keratin is degraded by microbes produce a specific protease keratinase. Many reports have been addressed to study Bacillus species as keratin-degrading microbes. However, most reported works were using pathogenic soil Bacillus. Our work has concentrated on keratinase produced bacterium, ...

Vimentin and keratin are coexpressed in many cells, but they segregate into two distinct intermediate filament (IF) networks. To understand the molecular basis for the sorting out of these IF subunits, we genetically engineered cDNAs encoding hybrid IF proteins composed of part vimentin and part type I keratin. When these cDNAs were transiently expressed in cells containing vimentin, keratin, o...

All known intermediate filament (IF) proteins display -8 -4 -1 a consensus sequence TYRKLLEGE at the carboxyl end of the rod domain. To analyse the contribution of this sequence to the formation of IF we have changed two of the invariant positions of this motif by site-directed mutagenesis. We produced three mutant keratins, each containing a single point mutation. Tyrosine at position -8 was c...

Barrett's esophagus is characterized by a distinct Th2-predominant cytokine profile (IL-4) from in vivo or ex vivo evidence. The detailed role of cytokines in Barrett's esophagus, particularly whether Th2 cytokines are causative factors driving metaplastic processes, remains unknown. In this study, air-liquid interface-cultured human esophageal epithelial cells were stimulated by a Th2 cytokine...

BACKGROUND Pachyonychia congenita (PC) is a group of autosomal dominant keratinizing disorders caused by a mutation in one of 4 keratin genes. Previous classification schemes have relied on data from case series and case reports. Most patients in these reports were not genetically tested for PC. OBJECTIVE We sought to clarify the prevalence of clinical features associated with PC. METHODS W...

The organization of the keratin intermediate filament cytoskeleton is closely linked to epithelial function. To study keratin network plasticity and its regulation at different levels, tools are needed to localize and measure local network dynamics. In this paper, we present image analysis methods designed to determine the speed and direction of keratin filament motion and to identify locations...

The molecular biology of these monogenetic skin diseases has been approached in two ways. The first was initiated by ultrastructural observations postulating mutations in the structural proteins of the cell so that linkage and keratin mutations could be extrapolated. The second approach has been by the use of transgenic mice technology to produce the phenotypes of human blistering skin diseases...