Clb2 mitotic cyclin inhibits cell cycle progression by preventing mitotic exit and DNA synthesis. To allow cell cycle progression, Clb2 proteolysis is triggered by Cdc20 during the metaphase-to-anaphase (M-A) transition and by Hct1 during mitotic exit and G1 [1-6]. A cis element called the destruction box is required for this proteolysis [7-11]. Recently, an additional cis element called the "K...

The BubR1 checkpoint protein performs multiple functions in mitosis. We have carried out a functional analysis of conserved motifs of human BubR1 (also known as BUB1B) and demonstrate that spindle assembly checkpoint (SAC) and chromosome attachment functions can be uncoupled from each other. Mutation of five proline-directed serine phosphorylation sites, identified in vivo by mass spectrometry,...

David W Mudge ([email protected]) Ken-Soon Tan ([email protected]) Rhianna Miles ([email protected]) David W Johnson ([email protected]) Scott B Campbell ([email protected]) Carmel M Hawley ([email protected]) Nicole M Isbel ([email protected]) Carolyn L Van Eps ([email protected]) David L N...

Rod Borup,* Jeremy Meyers,‡ Bryan Pivovar,† Yu Seung Kim,† Rangachary Mukundan,† Nancy Garland,§ Deborah Myers, Mahlon Wilson,† Fernando Garzon,† David Wood,† Piotr Zelenay,† Karren More,⊥ Ken Stroh,† Tom Zawodzinski, James Boncella,† James E. McGrath, Minoru Inaba,# Kenji Miyatake,∇ Michio Hori, Kenichiro Ota, Zempachi Ogumi,£ Seizo Miyata,+ Atsushi Nishikata,¶ Zyun Siroma,∞ Yoshiharu Uchimoto...