Fanconi anemia (FA) is a fatal genetic disorder associated with pancytopenia and cancer. Cells lacking functional FA genes are hypersensitive to bifunctional alkylating agents, and are deficient in DNA double-strand break repair. Multiple genes with FA-causing mutations have been cloned, however, the molecular basis for FA remains obscure. The results presented herein indicate that a Rad50-depe...

Peer-to-peer (P2P) overlay networks continue to evolve and grow to meet the challenges of a new age. Because peers can join or depart overlay networks at any time, researchers are particularly interested in how peers should be allowed to join overlay networks as well as how to minimize overhead in overlay networks. An Arrangement Graph-based Overlay (AGO) system can efficiently reduce system ov...

Data warehouses have emerged as a new business intelligence paradigm where data store and maintain in concurrent. The modifications are required in the implementation of Extract Transform Load (ETL) operations which now need to be executed in an online fashion. The adaptive approach takes two phases. The Extraction phase and the joining phase. The Extraction phase recognition of the subset of s...

A chromosomal segment with a unique structure around the immunoglobulin heavy chain joining region (JH) has been molecularly cloned from an Abelson murine leukemia virus-transformed cell line. Attached to JH3 in the cloned DNA, in inverted sequence, is the DNA from JH1 to the JH2 recognition sequence. The inverted segment is attached at its other end to the 5' recognition sequence of a diversit...

Sequence analysis of a large number of DNA clones containing a functional heavy chain variable, diversity, and joining (VHDJH) complex generated by VH to VHDJH joining (VH gene replacement) in the progeny derived from a common precursor cell transformed with a temperature-sensitive (ts) Abelson murine leukemia virus (A-MuLV) indicates that endogenous VH gene replacement in vitro generates immun...

In the presence of functional DNA repair pathways, DNA double-strand breaks (DSBs) are mainly repaired by non-homologous end-joining (NHEJ) or homologous recombination (HR), two conserved pathways that protect cells from aberrant chromosomal rearrangements. During the past two decades however, unusual and presumably distinct DNA end-joining repair activities have been unraveled in NHEJ-deficien...

This article describes a numerical study on the TOX-clinching process of the steel sheets. In addition, the influence of plastic anisotropy of the material on joining parameters is analyzed by evolution of the joint parameters such as undercut and neck thickness and punch force-displacement curve. Finite element analysis with ABAQUS/CAE-Explicit program is used to simulate two dimensional and t...

Polymerase-mediated recombination based on DNA polymerase chain reactions (PCRs) has been used to carry out directed joining at a present point of two DNA fragments initially contained in a plasmid and a single-stranded synthetic DNA. The process includes copying of these fragments by PCR with generation of an overlapping homologous region. Such overlap of 12 base pairs in length was found to b...