نتایج جستجو برای: its4
تعداد نتایج: 316 فیلتر نتایج به سال:
A total of twenty endophytic fungi successfully isolated from Melastoma malabathricum (Senduduk) were examined for their ability to decolourise azo dyes: Congo red, Orange G, and Methyl red and an anthraquinone dye, Remazol Brilliant Blue R. Initial screening on the glucose minimal media agar plates amended with 200 mg L(-1) of each respective dye showed that only isolate MS8 was able to decolo...
Out of fifty-two Iranian nonaflatoxigenic strains of Aspergillus flavus,collected from various substrates (soil and kernel) and sources (peanut, corn and pistachio), fifteen representatives were selected according to their different geographical origins (six provinces: Guilan and Golestan, Ardebil, Fars, Kerman and Semnan) and vegetative compatibility groups (VCGs, IR1 to IR15) for microsatelli...
Fusarium verticillioides is a pathogen of agriculturally important crops, especially maize. It is considered one of the most important pathogens responsible for fumonisin contamination of food products, which causes severe, chronic, and acute intoxication in humans and animals. Moreover, it is recognized as a cause of localized infections in immunocompetent patients and disseminated infections ...
Fungi belonging to the Cordyceps species have long been used as food and herbal medicines in Asia and are especially popular as commercially available powdered supplements. Despite this acceptance and use, little is known of the phylogenetic relationships of the genus. Presently, the neighbor-joining method based on the ITS1, 5.8S rRNA, and ITS2 regions was used to construct a phylogenetic tree...
BACKGROUND Lipases (acylglycerol acylhydrolase, E. C. 3. 1. 1. 3) are widely distributed among microorganisms, animals and plants, catalyzing the hydrolysis of triglycerides to free fatty acids and glycerol. Their commercial application includes pharmaceutical, chemical, and paper industries. OBJECTIVES This study aimed to isolate and screen lipolytic fungi from coastal waters of the southern...
OBJECTIVE To investigate the cytotoxic activity of endophytic fungi isolated from mangrove fungi. METHODS In the present study the DNA was isolated and the ITS region of 5.8s rRNA was amplified using specific primers ITS 1 and ITS4 and sequence was determined using automated sequencers. Blast search sequence similarity was found against the existing non redundant nucleotide sequence database ...
BACKGROUND Dermatophytes are the most common causative agents of superficial mycoses. Species identification of these fungi is important from therapeutic and epidemiological point of wive. Traditional approaches for identification of dermatophytes at the species level, relying on macroscopic and microscopic features of the colonies, usually are time-consuming and unreliable in many circumstance...
Background and Purpose Cryptococcus species are pathogenic and non-pathogenic basidiomycete yeasts that are found widely in the environment. Based on phenotypic methods, this genus has many species; however, its taxonomy is presently being re-evaluated by modern techniques. The Cryptococcus species complex includes two sibling taxa of Cryptococcus neoformans and Cryptococcus gattii. We aimed to...
کاندیدا از جمله شایعترین مخمرهای عفونت زای انسانی میباشد و در سالهای اخیر نرخ عفونتهای ایجاد شده توسط این مخمر افزایش یافته است. شناسایی زود هنگام کاندیداهای بیماری زا نقش مهمی را برای انجام اقدامات درمانی و کنترل عفونتهای بیمارستانی ایفا مینماید. روشهای سنتی شناسایی کاندیدا نسبت به روشهای مولکولی از حساسیت کمتری برخوردار بوده و به زمان بیشتری نیاز دارند. با توجه به وجود برخی محدودیتها...
به منظور ارزیابی تنوع ژنتیکی قارچ عامل بیماری آنتراکنوز گردو Ophiognomonia leptostyla در شمالغرب ایران تعداد 25 جدایه از مناطق مختلف جمعآوری و با استفاده از روش تک اسپورسازی خالص شدند. استخراج DNA از میسلیوم قارچ با استفاده از روش لیو و همکاران انجام شد. تکثیر منطقه ITS-rDNA توسط جفت آغازگر اختصاصی، ITS1 و ITS4 انجام گردید. همچنین منطقه IGS-rDNA با استفاده از جفت آغازگر NS1R و LR13R تکثیر ش...
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