The aim of this study was to evaluate the effectiveness of cleaning procedures for air-abraded zirconia after contamination with two silicone disclosing agents. Air-abraded zirconia ceramic specimens (IPS e.max ZirCAD) were contaminated with either GC Fit Checker white or GC Fit Checker II. Untreated zirconia specimens were used as control. Afterwards the surfaces were cleaned either with water...

Idescribe an assay for total cholesterol in serum, with use of the AutoAnalyzer ii (Technicon), in which cholesterol esters are saponified by alkali, cholesterol is held in aqueous micellar solution with a surfactant (Triton X-100) and oxidized by cholesterol oxidase, and the hydrogen peroxide produced is measured by chelation with Ti4+ and xylenol orange. An assay for free cholesterol in serum...

A human leukaemia cell line--HL-60--can be differentiated into neutrophils or macrophages and both differentiation processes are accompanied by changes of the lipid composition. Various methods were described for the extraction of lipids from cellular systems, but only two of them were applied to the HL-60 cell line so far. In this study we compared five selected extraction methods for the lipi...

UNLABELLED BACKGROUND Previously we have developed a butanol tolerant mutant of Clostridium acetobutylicum Rh8, from the wild type strain DSM 1731. Strain Rh8 can tolerate up to 19 g/L butanol, with solvent titer improved accordingly, thus exhibiting industrial application potential. To test if strain Rh8 can be used for production of high level mixed alcohols, a single secondary alcohol deh...

Methodological differences among laboratories are recognized as significant sources of variation in quantification of hair cortisol concentration (HCC). An important step in processing hair, particularly when collected from wildlife, is the choice of solvent used to remove or "wash" external hair shaft cortisol prior to quantification of HCC. The present study systematically compared methanol a...

Ciênc. Tecnol. Aliment., Campinas, 21(1): 10-13, jan.-abr. 2001 1 Recebido para publicação em 11/09/98. Aceito para publicação em 30/06/00. 2 Jahan Vegetable Oil Co., Research Department. 16 Daman Afshar st., wali-Assr Ave., Above wanak sq. Tehran 19697, Iran. SUMMARY Spent nickel catalyst (SNC) has the potential of insulting the quality of the environment in a number of ways. Its disposal has ...

Natural biopolymers from plant sources contain many impurities (e.g., fat, protein, fiber, natural pigment and endogenous enzymes), therefore, an efficient purification process is recommended to minimize these impurities and consequently improve the functional properties of the biopolymer. The main objective of the present study was to investigate the effect of different purification techniques...

BACKGROUND Some catheter-related bloodstream infections originate from catheter connectors; therefore, improved antisepsis of these might be expected to reduce the incidence of such infections. METHODS In this observational before/after study at a pediatric tertiary referral hospital, inpatients up to 16 years old undergoing hemopoietic stem cell transplants were studied. Catheter connection ...

Multiple solvent crystal structures (MSCS) of porcine pancreatic elastase were used to map the binding surface the enzyme. Crystal structures of elastase in neat acetonitrile, 95% acetone, 55% dimethylformamide, 80% 5-hexene-1,2-diol, 80% isopropanol, 80% ethanol and 40% trifluoroethanol showed that the organic solvent molecules clustered in the active site, were found mostly unclustered in cry...

Although a local modification of the usual alcohol-glycerinephenol-formaldehyde embalming formula has proved generally satisfactory through the years, continued price advances in the principal items of this formula led to its re-examination from a number of standpoints. A first consideration was the investigation of the possibility of substitutes for those items, such as ethanol and glycerine, ...