In vitro fertilisation (IVF) and related technologies are arguably the most challenging of all cell culture applications. The starting material is a single cell from which one aims to produce an embryo capable of establishing a pregnancy eventually leading to a live birth. Laboratory processing during IVF treatment requires open manipulations of gametes and embryos, which typically involves exp...

AIMS To compare the performance of the Sentinel blood culture system with two other systems for the recovery of streptococci and anaerobes. METHODS Blood cultures were taken from 55 patients one to two minutes after dental extraction. The samples were tested by the radiometric Bactec 460; the Isolator 10, which works by lysis centrifugation; and Sentinel, a fully automated system, which detec...

BACKGROUND There is considerable interest in investigating the role of the microbiota in various diseases, including transplant rejection. Germ-free (GF) and gnotobiotic mice are powerful models for this line of investigation, but performing surgery within the confines of a sterile housing isolator is exceptionally challenging. Development of rigorous protocols to be able to remove axenic mice ...

We retrospectively compared the utility of a fungal isolation device (Isolator) versus conventional techniques for recovering fungal organisms from blood cultures obtained from neutropenic cancer patients. Positive cultures were deemed true pathogens, possible pathogens, or contaminants according to laboratory and clinical criteria. Fifty-three patients had 66 positive blood cultures for fungi,...

To encourage the utilization of the isolation chamber as a research tool, the cost of its use should be lowered. Methods and devices must be developed which make more efficient use of the space within the isolator and allow the operator to work more effectively in this confined area. A simple vacuum-cleaning system is described; it consists of a nozzle and flexible hose which connect through th...

Driving on an analogy with the technique of composite pulses in quantum physics, we theoretically propose a broadband Faraday rotator and thus a broadband optical isolator, which is composed of sequences of ordinary Faraday rotators and achromatic quarter-wave plates rotated at the predetermined angles.

A bimodal planar waveguide segment of specific length and thickness between two thinner single mode sections can serve as an interferometer. Depending on the phase gain of the two modes in the thick region, these fields can interfere destructively or constructively at the transition from the bimodal to the single mode section. We employ this geometry to realize a simple magnetooptic isolator co...

The Toucher–Isolator game was introduced recently by Dowden, Kang, Mikalački and Stojaković. Two players, Toucher Isolator, alternately claim an edge from a graph, where plays first. aims to touch as many vertices possible, Isolator minimize the number of that are so touched. Among trees with n vertices, they showed star is best choice for asked most suitable tree Toucher. Later, Räty answer pa...