We present a new polymerase chain reaction assay based on telomeric sequences of Leishmania donovani. When this assay was used in dilutions of purified L. donovani DNA, a strong amplification signal was observed with 1 fg of DNA. In a specificity test that used purified DNA from Old World and New World Leishmania, the assay recognized all parasites isolated from patients with visceral leishmani...

A gram-negative coccobacillus was isolated from the lower respiratory tract of a cat with chronic obstructive pulmonary disease. The isolate required CO2 and V factor for growth and was initially identified as Haemophilus paraphrophilus on the basis of its nutritional requirements, colony morphology, and some biochemical tests. Because of the host specificity of Haemophilus species and discrepa...

A murine monoclonal antibody (MAb) with human CD4 specificity was tested for the ability to inhibit primary human immunodeficiency virus type 1 (HIV-1) isolates clades A through E. Human peripheral blood mononuclear cells (PBMC) were used as target cells for infectivity. The HIV-1 primary isolates were examined for the capacity to infect PBMC targets in the presence or absence of the anti-CD4 M...

conclusions obtained data demonstrated that the designed assay is an accurate, easy, rapid, and cost-effective tool for detecting v. cholerae that can serve as an alternate to current bacteriological tests. background several epidemic and endemic cases have been reported involving vibrio cholerae (v. cholerae) as a causative organism in serious diarrheal diseases with high mortality. hence, qui...

Recent associative models of cognitive control hypothesize that cognitive control can be learned (optimized) for task-specific settings via associations between perceptual, motor, and control representations, and, once learned, control can be implemented rapidly. Midfrontal brain areas signal the need for control, and control is subsequently implemented by biasing sensory representations, boost...

Antigen detection rapid diagnostic tests have been developed for first-line large-scale screening given their rapidity, simplicity, and accuracy. This study evaluates the performance of an antigen test (BLOK BioScience, London, UK) detecting SARS-CoV-2 nucleocapsid protein. Serially diluted isolate 110 NPS from COVID-19 patients were tested to determine test’s sensitivity, other viral isolates ...

In this study, a selective and specific sorbent for radium extraction was developed the first time using ion-imprinted polymer technology. A novel original approach used in order to screen best synthesis conditions. After identifying monomer candidates, porogen, complexation from electrospray ionization mass spectrometry experiments, solubility tests, conductimetry five polymers (IIPs) were syn...

background: the objective of this study was to determine the diagnostic value of magnetic resonance imaging for shoulder joint pathologies and then compare the results with arthroscopy, the standard for joint diagnosis. methods: in this cross-sectional study, 80 patients with shoulder joint disorders, who underwent final arthroscopy, were studied. based on patients’ medical history and physical...