Mouse embryonic stem cells (ESCs) and induced pluripotent stem (iPS) cells can be used as models of neuronal differentiation for the investigation of mammalian neurogenesis, pharmacological testing, and development of cell-based therapies. Recently, mouse iPS cell lines have been generated by Sleeping Beauty (SB) transposon-mediated transgenesis (SB-iPS). In this study, we determined for the fi...

Adult cells have been reprogrammed into induced pluripotent stem (iPS) cells by introducing pluripotency-associated transcription factors. Here, we discuss recent advances and challenges of in vitro reprogramming and future prospects of iPS cells for their use in diagnosis and cell therapy. The generation of patient-specific iPS cells for clinical application requires alternative strategies, be...

background : in anterior endodontically treated teeth a better esthetic can be achieved by using all-ceramic post -core restorations instead of metal post systems. maximum retention and fracture resistance of the post are the criteria upon which selection of a post system is based. the aim of this study was to evaluate the fracture resistance and retention of two all-ceramic post and core syste...

Rural households in India rely extensively on informal biomedical providers, who lack valid medical qualifications. Their numbers far exceed those of formal providers. Our study reports on the education, knowledge, practices and relationships of informal providers (IPs) in two very different districts: Tehri Garhwal in Uttarakhand (north) and Guntur in Andhra Pradesh (south). We mapped and inte...

OBJECTIVE Endothelial damage is an early requisite step for atherosclerosis after vascular injury. It has been reported that vascular wall cells can develop from induced pluripotent stem (iPS) cell-derived fetal liver kinase-1-positive (Flk-1(+)) cells. Here, we investigated the efficacies of intravenously administered iPS cell-derived Flk-1(+) cells on reendothelialization and neointimal hyper...

Long intervening non-coding RNAs (lincRNAs) are increasingly being implicated as important factors in many aspects of cellular development, function, and disease, but remain poorly understood. In this study, we examine the human retinal pigment epithelium (RPE) lincRNA transcriptome using RNA-Seq data generated from human fetal RPE (fRPE), RPE derived from human induced pluripotent stem cells (...

Cyber attacks are continuing to hamper working of Internet services despite increase in the use of network security systems such as, firewalls and Intrusion protection systems (IPS). Recent Denial of Service (DoS) attack on Independence Day weekend, on July 4th, 2009 launched to debilitate the US and South Korean governments’ websites is indicative of the fact that the security systems may not ...

After the reviews of : Mr Atilla Baskurt Professor, INSA Lyon Mr Alberto Del Bimbo Professor, University of Firenze The dissertation is approved by the following members of the Final Oral Committee : Ms Régine André-Obrecht Professor, University Paul Sabatier Committee chair Mr Atilla Baskurt Professor, INSA Lyon Thesis referee Ms Jenny Benois-Pineau Professor, University Bordeaux 1 Thesis supe...

BACKGROUND Liver injuries are important medical problems that require effective therapy. Stem cell or hepatocyte transplantation has the potential to restore function of the damaged liver and ameliorate injury. However, the regulatory factors crucial for the repair and regeneration after cell transplantation have not been fully characterized. Our study investigated the effects and the expressio...

Telomere reprogramming and silencing of exogenous genes have been demonstrated in mouse and human induced pluripotent stem cells (iPS cells). Pigs have the potential to provide xenotransplant for humans, and to model and test human diseases. We investigated the telomere length and maintenance in porcine iPS cells generated and cultured under various conditions. Telomere lengths vary among diffe...