Antisera produced in rabbits immunized with an enteroinvasive O143 strain of Escherichia coli were absorbed with an avirulent derivative of the same strain. The resulting sera have been previously shown to recognize enteroinvasive pathogens when used in an enzyme-linked immunosorbent assay. In the current study, Western blots (immunoblots) showed that such an absorbed rabbit antiserum recognize...

1 University of Toledo, 2801 West Bancroft Street, Toledo, OH 43606, USA 2 Max-Planck-Institut für Astronomie, Königstuhl 17, D-69117 Heidelberg, Germany 3 Steward Observatory, University of Arizona, 933 North Cherry Avenue, Tucson, AZ 85721, USA 4 National Radio Astronomy Observatory, 520 Edgemont Road, Charlottesville, VA 22903, USA 5 NHSC/IPAC/Caltech, 770 South Wilson Avenue, Pasadena, CA 9...

Single injections of morphine induce a state of acute opioid dependence in humans and animals, measured as precipitated withdrawal when an antagonist is administered 4-24 h after morphine. Additional morphine exposure at daily or weekly intervals results in further increases in withdrawal severity, suggesting that acute opioid dependence reflects the early stages in the development of a chronic...