The role of calcium in transducing the signal for interleukin-1 (IL-1) secretion was examined in the MQ-NCSU chicken macrophage cell line. Cells were maintained in RPMI 1640 medium supplemented with 5% chicken serum and antibiotic-antimycotic solution at 40 C and 5% CO2. The effects of stimulation with lipopolysaccharide (LPS), calcium ionophore A23187, or a combination of both on IL-1 secretio...

Facilitated SO4(2-) transfers by hydrogen bond-forming ionophores are investigated across the nitrobenzene (NB)-water interface by using polarography with a dropping electrolyte electrode. Bis-thiourea 1, alpha,alpha'-bis(N'-p-nitrophenylthioureylene)-m-xylene, is found to significantly facilitate the transfer of the highly hydrophilic SO4(2-) whereas its counterpart, N-(p-nitrophenyl)-N'-propy...

BACKGROUND Eosinophils from asthmatic patients are known to release greater amounts of leukotrienes than normal eosinophils when stimulated by the calcium ionophore A23187. The effect of platelet activating factor (PAF) in priming eosinophils was investigated. METHODS Eosinophils were obtained from 18 asthmatic patients and 18 healthy donors. Cells separated by the Percoll gradients were incu...

The effect of the ionophore A-23187 was tested on isolated secretory granules of rat parotid gland. The ionophore caused extensive release of calcium from the granules without effecting release of amylase or other secretory proteins. It is therefore concluded that the role of calcium in the granules is probably not that of a stabilizing agent.

BACKGROUND In vitro maturation (IVM) of human oocytes is an emerging procedure quickly incorporated into the world of assisted reproductive technologies. As an effective method of in vitro maturation, several studies have reported the critical role of differentions on activating the complex process involved in both gamete maturation and fertilization. OBJECTIVE In this study, we supplemented ...

Scorpion toxin enhances activation of the action potential Na+ ionophore by the alkaloid neurotoxins veratridine, batrachotoxin, and aconitine. The pure toxin can be iodinated in a lactoperoxidase-catalyzed reaction. Both the monoiodoand diiodotoxin derivatives retain physiologic activity. Both monoiodoand diiodoscorpion toxin bind to a single class of saturable binding sites in electrically ex...

a new poly(vinylchloride) membrane sensor for cr3+ ions based on di (benzylamino)glyoxime asan ionophore was prepared. the electrode has a linear dynamic range 1×10-6-1×10-1 mol l-1,with anernstian slope of 20.3 ± 0.5 mv per decade and a detection limit of 2×10-7. it has a fast responsetime of

Metrological properties of voltammetric electrodes, in the situation where on their surface an electrochemical reaction of oxidizing/reduction takes place, were analyzed in this chapter. The properties of electrodes on which a reaction controlled by ion transport process takes place were taken into consideration. Also, it was analyzed how the electrode’s shape and the voltage polarizing the ele...