K E Y W O R D S T-WaveTM ion mobility, IMS, MS, MS, structure elucidation A P P L I C AT IO N B E N E F I T S This application note illustrates how multi-stage mass spectra up to MS can be generated on a SYNAPT G2 HDMS System in a single experiment. This experiment avoids the need to trigger on, isolate, and fragment specific ions, as is done in directed MS methods. Moreover, the duty cycle rem...

Understanding the 3-D structure and dynamics of proteins and other biological macromolecules in various environments is among the central challenges of chemistry. Electrospray ionization can often transfer ions from solution to gas phase with only limited structural distortion, allowing their profiling using mass spectrometry and other gas-phase approaches. Ion mobility spectrometry (IMS) can s...

A pyrolysis-gas chromatography-ion mobility spectrometry (Py-GC-IMS) briefcase system has been shown to detect and classify deliberately released bioaerosols in outdoor field scenarios. The bioaerosols included Gram-positive and Gram-negative bacteria, MS-2 coliphage virus, and ovalbumin protein species. However, the origin and structural identities of the pyrolysate peaks in the GC-IMS data sp...

Three disease phenotypes, Barrett's esophagus (BE), high-grade dysplasia (HGD), esophageal adenocarcinoma (EAC), and a set of normal control (NC) serum samples are examined using a combination of ion mobility spectrometry (IMS), mass spectrometry (MS), and principal component analysis (PCA) techniques. Samples from a total of 136 individuals were examined, including 7 characterized as BE, 12 as...

Low field ion mobility spectrometry-mass spectrometry (IMS-MS) techniques exhibit low orthogonality, as inverse mobility often scales with mass to charge ratio. This inadequacy can be mitigated by adding vapor dopants, which may cluster with analyte ions and shift their mobilities by amounts independent of both mass and mobility of the ion. It is therefore important to understand the interactio...

The purpose of the current study was to explore and assess the potential of high resolution electrospray ionization atmospheric pressure ion mobility spectrometry (ESI–AP-IMS) as a field analytical method for the detection and identification of mixtures of sulfonylurea (SU) herbicides in aqueous samples. Because of increased usage, persistent behavior, and potential for crop damage, an environm...

Here we report the first High Kinetic Energy-Ion Mobility Spectrometry-Mass Spectrometric (HiKE-IMS-MS) investigations involving four fluranes; isoflurane, enflurane, sevoflurane and desflurane. Unlike standard (atmospheric pressure) IMS, HiKE-IMS can detect these compounds in positive ion mode. This is because its low-pressure environment (∼ 14 mbar) associated short drift times ensure reagent...

In this paper, a novel approach based on paper spray ionization coupled with ion mobility spectrometry (PSI-IMS) was developed for rapid, in situ detection of cocaine residues in liquid samples and on various surfaces (e.g. glass, marble, skin, wood, fingernails), without tedious sample pretreatment. The obvious advantages of PSI are its low cost, easy operation and simple configuration without...

Improvements were made to a pyrolysis-gas chromatography-ion mobility spectrometry (Py-GC-IMS) stand-alone biodetector to provide more pyrolyzate compound information to the IMS detector module. Biological aerosols were disseminated at DRES, Alberta, Canada and the Py-GC-IMS was tested for its ability to detect the biological aerosols. Forty-two trials were conducted and a simple area calculati...