To analyze the mechanism of integral membrane protein localization in the early Golgi apparatus of Saccharomyces cerevisiae, we have used Och1p, a cis-Golgi mannosyltransferase. A series of influenza virus hemagglutinin (HA) epitope-tagged fusion proteins was constructed in which invertase is appended to the Golgi-luminal carboxy terminus of full-length Och1p. Several constructs included a Kex2...

Alkaline invertase was induced during the initiation of suspension cultures of single cells from leaf explants of sugar beets in Murashige-Skoog liquid medium which contained benzyladenine. This activity was barely detectable in the leaves themselves. In suspension cultures, the presence of both acid and alkaline invertases was detected; alkaline invertase was only present in the cytoplasm of t...

In tissue slices from rapidly expanding internodes of sugar cane the vacuolar invertase level is a function of the balance between synthesis and destruction. The enzyme is destroyed in the tissue at an approximately constant rate with a half time of 2 hours. Invertase synthesis is regulated by both auxin and glucose. From studies with inhibitors of protein and RNA synthesis we conclude that aux...

Imimiature interniodes of sugar canie contain an aci(l in% ertase witlh optinmuiim activity between pH 5.0 and 5.5 (1.2). The level of this enzynme shovws marke(d seasonal variation, being high w-hen growth is rapid an(l low otherwise. Acidl invertase is absenit fromii mature interno(les of sugar cane varieties wvith a high capacity for sugar storage, whiclh instea(l containi a neutral invertas...

A laboratory experiment has been conducted to investigate the ecological toxicity of flubendiamide and spinosad at their recommended field rates and higher rates (1.0, 2.5, 5.0, 7.5, 10.0 kg ha-1) on cellulase, invertase and amylase in black and red clay soils after 10, 20, 30 and 40-day exposure under controlled conditions in groundnut (Arachis hypogaea L.) soils of Anantapur District, Andhra ...

An acid invertase was isolated and partially purified from mature sugarcane (var HSF 240) stem juice by a combination of Ammonium sulphate, DEAE-cellulose and gel filtration. The purified acid invertase had a specific activity of 17.05 Umg1. Invertase was characterized for various parameters. The pH and temperature optima of the enzyme were 3.0 and 45°C respectively. The Km value and energy of ...

THE phenomenon of invertase-acceleration by serum was first observed in the course of a biochemical investigation into the causal factors of an experimental immunity produced against the myiasis-producing larva of Cordylobia anthropophaga [Blacklock, Gordon and Fine, 1930]. Although this phenomenon was of no interest from the point of view of the inquiry that led to its discovery, I became inte...

Invertase activity in wild-type and several mutant strains of Neurospora crassa, was significantly enhanced by supplementing the culture medium with cyclic AMP (5.0 mM). Cyclic AMP stimulated invertase by (i) increasing overall enzyme production and (ii) substituting for natural carbohydrate inducers (i.e. galactose or raffinose) as a requirement for maximal enzyme production. The effect of cAM...