The intrinsic protein fluorescence of the polygalacturonase from Colletotrichium lindemuthianum was exploited in stopped-flow experiments aimed at elucidating the kinetic mechanism for this enzyme. Binding of the polymeric substrate polygalacturonic acid (PGA) essentially produced a triphasic fluorescence profile. There was an initial rapid quench in fluorescence, consistent with the rapid form...

Title of Document: THE TRANSCRIPTIONAL REGULATOR MPHR(A): CHARACTERIZATION AND CIRCUIT ENGINEERING Adam Smolinsky, Master of Science, 2008 Directed By: Dr. T. Ashton Cropp, Assistant Professor, Department of Chemistry and Biochemistry The MphR(A) transcriptional repressor protein was incorporated into high-efficiency expression vectors, purified and characterized. Initial screens for crystallog...

Equilibrium dialysis studies on the binding of the blood group B-specific Bandeiraea simplicifolia lectin to methyl alpha-D-galactopyranoside demonstrated the existence of one carbohydrate binding site per subunit for the tetrameric protein, with an intrinsic association constant of 8.6 x 10(4) (M) (-1) at 2 degrees and 3.3 x 10(4) (M)(-1) at 20 degrees. These values correspond to a free energy...

The water relations play a fundamental role in seed comprehension of biology. In order to describe the process of drying and the effect on water activity, which controls biological change in storage, a sound knowledge of the relationship between equilibrium moisture content (EMC) and water activity/equilibrium relative humidity (ERH) is essential. The relationship between the total moisture con...

– Non-equilibrium dynamics in a Ag(Mn) spin glass are investigated by measurements of the temperature dependence of the remanent magnetisation. Using specific cooling protocols before recording the thermo-or isothermal remanent magnetisations on reheating , it is found that the measured curves effectively disclose non-equilibrium spin glass characteristics such as ageing and memory phenomena as...

The incorporation of CO-heme into single bilayer, egg lecithin vesicles was examined by following the spectral changes that occur when the porphyrin becomes embedded in the membranes. The rate of CO-heme uptake by liposomes is extremely fast (t1/2 less than or equal to 20 ms at 10 degrees C), and the maximum extent is roughly 1 heme/5 phospholipid molecules. This limiting stoichiometry is due t...

Abstract Non-equilibrium quasiparticles are possible sources for decoherence in superconducting qubits because they can lead to energy decay or dephasing upon tunneling across Josephson junctions (JJs). Here, we investigate the impact of intrinsic properties two-dimensional transmon on quasiparticle (QPT) and discuss how use dynamics gain critical information about quality JJ barrier. We find r...

The reversible photoswitching of the photochromic fluorescent protein Padron0.9 involves a cis-trans isomerization of the chromophore. Both isomers are subjected to a protonation equilibrium between a neutral and a deprotonated form. The observed pH dependent absorption spectra require at least two protonating groups in the chromophore environment modulating its proton affinity. Using femtoseco...