To examine the effect of inclusion insoluble nonstarch polysaccharides (iNSP) in weaned diets on postweaning piglet performance, intestinal activity, and microbial composition 2 experiments were designed. In Exp. 1, 180 piglets were weaned at 28 d of age and divided over 3 treatments: positive control (PC; highly digestible protein), negative control (NC; standard cereal-based diet), and an exp...

HIV-1 infection is associated with an early and profound depletion of mucosal memory CD4+ T cells, a population that plays an indispensable role in the regulation of isotype switching and transepithelial transport of antibodies. In this study, we addressed whether the depletion of CD4+ T cell in HIV-1-infected individuals results in altered humoral responses specific to antigens encountered at ...

A longstanding hypothesis in intestinal microbial ecology is that autochthonous microbes (resident) play a role that is distinct from allochthonous microbes (transient microbes in the fecal stream). A challenge has been to identify this pool of microbes. We used laser capture microdissection to collect microbes from the mouse ascending colon. This area contains transverse folds that mimic human...

A sobering fact is that human DNA represents <10% of the total DNA within each of us. Every mucosal surface harbors a diverse and distinct microbial composition, with the human intestines containing by far the largest and most complex microbial ecosystem (intestinal microbiota). Serving as the filter for our largest environmental exposure—what we eat—the human intestinal microbiota is enormous—...

Beneficial microbes are responsible for the synthesis of nutrients and metabolites that are likely important for the maintenance of mammalian health. Many nutrients and metabolites derived from the gut microbiota by luminal conversion have been implicated in the development, homeostasis and function of innate and adaptive immunity. These factors clearly suggest that intestinal microbiota may in...

Different dietary fat and energy subtypes have an impact on both the metabolic health and the intestinal microbiota population of the host. The present study assessed the impact of dietary fat quality, with a focus on dietary fatty acid compositions of varying saturation, on the metabolic health status and the intestinal microbiota composition of the host. C57BL/6J mice (n 9-10 mice per group) ...

this study was conducted to evaluate the effect of camphor levels on performance, organ weight, testosterone and thyroid hormones, intestinal selective bacteria, and meat peroxidation of japanese quail. two-hundred 1-d chicks were allocated in 20 cages with five treatments and four replicates (10 chicks per each cage) using complete randomized block design. treatments were including: 0, 62.5, 1...

This study hypothesized that the gut microbial populations, intestinal morphology, and cytokine production are differentially altered in 2 different pig breeds, namely, Chinese native Jinhua pigs and European Landrace pigs, after orally challenge with enterotoxigenic Escherichia coli (ETEC) K88. A total of 12 Jinhua pigs and 12 Landrace pigs were allocated to either the nonchallenged or the cha...

a total of 300 one-day old male broilers (cobb 500) were randomly divided into four treatment groups. the groups comprised of 15 birds each, and were defined by diet and the birds in each group were fed 0 (control), 3, 5, or 7 kgy electron-beam irradiated diets. a total of 5 replicates were performed. the chemical composition of the diet was not affected by irradiation.irradiation doses of 5 an...

Sustaining a balanced intestinal microbial community is critical for maintaining intestinal health and preventing chronic inflammation. The gut is a highly dynamic environment, subject to periodic waves of peristaltic activity. We hypothesized that this dynamic environment is a prerequisite for a balanced microbial community and that the enteric nervous system (ENS), a chief regulator of physio...