An inorganic phosphate (P(i))-restricted diet is important for patients with chronic kidney disease and patients on hemodialysis. Phosphate binders are essential for preventing hyperphosphatemia and ectopic calcification. The sodium-dependent P(i) (Na/P(i)) transport system is involved in intestinal P(i) absorption and is regulated by several factors. The type II sodium-dependent P(i) transport...

Eight strains isolated from the stems of Lippia sidoides were identified as belonging to Lactococcus lactis, a bacterial species considered as "generally recognized as safe". Their capacity to solubilize/mineralize phosphate was tested in vitro with different inorganic and organic phosphorus (P) sources. All strains were able to solubilize calcium phosphate as an inorganic P source, and the bes...

By T. J. GREENWALT AND V. E. AYERS T HE DISTRIBUTION of phosphate compounds in acid extracts of the erythrocytes of infants has heretofore been investigated only by fractional analysis for inorganic, total and hydrolyzable phosphates.1 In our previous work hydrolysis of trichloracetic acid extracts of cord blood erythrocytes was used to characterize the following fractions : ( 1 ) labile phosph...

A cDNA encoding 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase was isolated from a Spinacia oleracea leaf library and used to express a recombinant enzyme in Escherichia coli and Spodoptera frugiperda cells. The insoluble protein expressed in E. coli was purified and used to raise antibodies. Western blot analysis of a protein extract from spinach leaf showed a single band of 90.8 kDa. So...

We describe an enzymatic method for determining inorganic phosphate in serum and urine, with use of the “CentrifiChem.” The sample is mixed with a combined enzyme-substrate system consisting of the enzymes phosphorylase a, phosphoglucomutase, and glucose-6-phosphate dehydrogenase, the coenzyme NADP, the substrate glycogen, and adenosine 5’-monophosphate as the activator for phosphorylase. The i...

Oxidation of substituted imidazoles with modified Fenton's reagent (Fe(II), ascorbic acid, EDTA and H2O2) in aqueous solution at pH 5 -7 in the presence of inorganic orthophosphate gives high yields of an unknown bound phosphate, in the case of 2-methylimidazole up to 3 5 % based on inorganic phosphate. The dependence of the yields on reaction parameters and the hydrolytic behaviour of the comp...

Because previous studies have demonstrated that renal inorganic phosphate reabsorption is enhanced in rats after dietary phosphorus deprivation, we studied the effects of parathyroid hormone (PTH) upon inorganic phosphate reabsorption in acutely thyroparathyroidectomized rats stabilized on a low phosphorus diet to determine if the phosphaturic response to PTH is impaired during phosphorous depl...

The effects of acute i.v. inorganic phosphate (Pi) loading were studied on nembutalized mongrel dogs previously subjected to unilateral splanchnicotomy ("renal denervation"). GFR (51Cr-EDTA) was not different on the intact and the denervated side, while urine output (V), sodium excretion (UNaV), and urinary excretion (UPiV) of inorganic phosphate of denervated kidneys were significantly increas...

In the erythrocytes of a patient with hereditary nonspherocytic hemolytic anemia, a homozygous expression of hexokinase deficiency was detected. The mutant enzyme was characterized by normal kinetic parameters with respect to its substrates, glucose and MgATP2-, normal pH optimum, normal heat stability at 40 degrees C, but abnormal behavior with respect to its regulation by glucose-1,6-diphosph...

Cellular metabolism depends on the appropriate concentration of intracellular inorganic phosphate; however, little is known about how phosphate concentrations are sensed. The similarity of Pho84p, a high-affinity phosphate transporter in Saccharomyces cerevisiae, to the glucose sensors Snf3p and Rgt2p has led to the hypothesis that Pho84p is an inorganic phosphate sensor. Furthermore, pho84Delt...