Data are presented to demonstrate that the circumpulpal dentine of deciduous teeth can be used to reconstruct a detailed record of childhood exposure to lead. By combining high spatial resolution laser ablation ICP-MS with dental histology, information was acquired on the concentration of lead in dentine from in utero to several years after birth, using a true time template of dentine growth. T...

OBJECTIVE: To investigate and compare the protective impact of the in situ formed salivary pellicle on enamel and dentine erosion caused by different acids at pH 2.6. METHODS: Bovine enamel and dentine samples were exposed for 120 min in the oral cavity of 10 healthy volunteers. Subsequently, enamel and dentine pellicle-covered specimens were extraorally immersed in 1 ml hydrochloric, citric or...

OBJECTIVES This study aimed to analyse the erosion-inhibiting potential of a single application of stannous chloride-containing fluoride solution on pellicle-covered enamel and dentine under constant acid flow conditions in vitro. DESIGN Bovine enamel (n=60) and dentine (n=60) samples were exposed 1h to the oral cavity of 4 healthy volunteers to allow for in situ pellicle formation. Pellicle-...

Rat calvaria cells (RC cells) and monkey periodontal ligament fibroblasts (MPL cells) were cultured for 4-6 days with demineralized dentine, acid-washed glass particles or untreated glass particles to determine whether the presence of these particles can affect the orientation of the cells. Encapsulation of dentine particles began as early as the first day in culture and occasional cells were a...

The aim of this study was to assess the validity of micro-spatial dentine lead (Pb) levels as a biomarker for accurately estimating exposure timing over the prenatal and early childhood periods and long-term cumulative exposure to Pb. In a prospective pregnancy cohort sub-sample of 85 subjects, we compared dentine Pb levels measured using laser ablation-inductively coupled plasma mass spectrome...

AIM To evaluate the remineralization ability of two endodontic sealer cements. METHODOLOGY Mid-coronal dentine surfaces were subjected to: (i) 37% phosphoric acid (PA) or (ii) 0.5 mol L-1 ethylenediaminetetraacetic acid (EDTA) conditioning prior to the application of two experimental hydroxyapatite-based cements, containing sodium hydroxide (calcypatite) or zinc oxide oxiapatite respectively....

OBJECTIVE This in vitro study aimed to investigate the preventive effect of brushing with anti-erosive toothpastes compared to a conventional fluoride toothpaste on dentine erosion. MATERIALS AND METHODS Bovine dentine specimens (n=12 per subgroup) were eroded in an artificial mouth (6 days, 6×30 s/day) using either citric acid (pH:2.5) or a hydrochloric acid/pepsin solution (pH:1.6), simulat...

OBJECTIVES To compare the micro-tensile bond strengths of two different adhesive systems (ABF (Clearfil Protect Bond), Kuraray Medical Inc., Tokyo, Japan) and Prime & Bond NT (PBNT, Dentsply, Konstanz, Germany) bonded to caries-affected dentine retained after chemo-mechanical caries removal using Carisolv gel, with that retained after excavation using conventional hand instrumentation. METHOD...

Aim: The aim of the present studywas to compare the effect of a calcium sodium phosphosilicate (NovaMin®) desensitizing agent (a bioactive glass) on both cervical and midcoronal dentine sections using scanning electron microscopy (SEM). Methods: Sections of both cervical and mid coronal dentine were prepared and etched with 5% citric acid for 5 minutes; these sections were then brushed with 2mg...