BACKGROUND Exposure to dioxins results in a broad range of pathophysiological disorders in human fetuses. In order to evaluate the effects of dioxins on the feto-placental tissues, we analyzed the gene expression in 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) treated primary cultures of human amniotic epithelial cells. METHODS Human amniotic epithelial cells were dispersed by trypsin from amni...

LPS binding to Toll-like receptor 4 induces a large number of genes through activation of NF-kappaB and IFN-regulatory factor-3 (IRF-3). However, no previous reports have tested the role of serum proteins in LPS-induced gene expression profiles. To investigate how serum proteins affect LPS-induced signaling, we investigated LPS-inducible genes in PBMC using an oligonucleotide probe-array system...

Microbial products such as LPS stimulate macrophages to produce a wide diversity of inducible gene products needed for immediate host defense and priming of an appropriate acquired immune response. In this study, we have examined LPS-inducible gene expression in subclones of a mouse macrophage cell line, RAW264, using cDNA microarrays. Even archetypal target genes such as TNF-alpha were not ind...

results this study shows the prepared gene construct is inducible by glucose. gene expression of preproinsulin was observed in muscle tissue of rabbits. conclusions these finding indicated that research in diabetes mellitus gene therapy could be performed on larger animals. objectives the purpose of this study was to construct glucose inducible insulin gene plasmid and use it in the muscle tiss...

Salicylic acid (SA) is known to be involved in the immunity against Clavibacter michiganensis ssp. (Cmm) that causes bacterial canker tomato. To identify candidate genes associated with SA-inducible Cmm resistance, transcriptome analysis was conducted via RNA sequencing tomato plants treated SA. SA treatment upregulated various defense-associated genes, such as PR and GST cotyledons. A comparis...

Aluminum (Al) rhizotoxicity is one of the major environmental stresses that decrease global food production. Clarifying the molecular mechanisms underlying Al tolerance may contribute to the breeding of Al-tolerant crops. Recent studies identified various Al-tolerance genes. The expression of these genes is inducible by Al. Studies of the major Arabidopsis thaliana Al-tolerance gene, ARABIDOPSI...

Plants respond to survive under water-deficit conditions via a series of physiological, cellular, and molecular processes culminating in stress tolerance. Many drought-inducible genes with various functions have been identified by molecular and genomic analyses in Arabidopsis, rice, and other plants, including a number of transcription factors that regulate stress-inducible gene expression. The...

A transcription factor DREB2A functions as a key regulator not only in drought stress responses but also in heat stress (HS) responses, and activates expression of many abiotic stress-responsive-genes involved in drought and HS tolerance. HsfA3 is one of the most up-regulated heat-inducible genes in transgenic plants overexpressing DREB2A. In this study, the analyses of HsfA3 expression profile...

Objective(s): Fluvoxamine is a well-known selective serotonin reuptake inhibitor (SSRI); Despite its anti-inflammatory effect, little is known about the precise mechanisms involved. In our previous work, we found that IP administration of fluvoxamine produced a noticeable anti-inflammatory effect in carrageenan-induced paw edema in rats. In this study, we aimed to evaluate the effect of fluvoxa...

Rrd1p (resistance to rapamycin deletion 1) has been previously implicated in controlling transcription of rapamycin-regulated genes in response to rapamycin treatment. Intriguingly, we show here that Rrd1p associates with the coding sequence of a galactose-inducible and rapamycin non-responsive GAL1 gene, and promotes the association of RNA polymerase II with GAL1 in the absence of rapamycin tr...