Valerian (Valeriana officinalis L.) is an important medicinal plant that is widely exploited for its roots and rhizomes which contain valepotriates and valerenic acids (with putative pharmacological activities). Thus root proliferation of valerian is very important. The aim of this study was to establish a practical tissue culture method for rapid and large-scale induction of V. officinalis L. ...

The ability of indole derivatives to facilitate RNA polymerase transcription of the L-arabinose operon in Escherichia coli was shown to require the catabolite activator protein (CAP) as well as the araC gene product. Adenosine 3',5'-monophosphate (cAMP) was not obligatory for araBAD transcription when the cells were grown in the presence of 1 mM indole-3-acetic acid or in the presence of indole...

An efficient system was established for high frequency embryogenesis and regeneration of indica rice, Oryza sativa L. cv. MDU 5. Basic media, carbohydrate sources and concentrations, agar concentrations, amino acids, cytokinins and auxins were evaluated in callus induction and regeneration media to establish the most efficient regeneration protocol for MDU 5 indica rice. The optimized callus in...

Indole-3-acetic acid (plant auxin) has low toxicity but dramatically enhances the killing of mammalian cells on illuminating phenothiazinium dyes with red light. Suitable dyes include toluidine blue, used in cancer diagnosis because of localization in tumors, and methylene blue, used in experimental photodynamic therapy of cancer. The photosensitized oxidation of indole acetic acid forms a free...

Transcription of the Azospirillum brasilense ipdC gene, encoding an indole-3-pyruvate decarboxylase involved in the biosynthesis of indole-3-acetic acid (IAA), is induced by IAA as determined by ipdC-gusA expression studies and Northern analysis. Besides IAA, exogenously added synthetic auxins such as 1-naphthaleneacetic acid, 2,4-dichlorophenoxypropionic acid, and p-chlorophenoxyacetic acid we...

Indole-3-acetic acid (plant auxin) has low toxicity but dramatically enhances the killing of mammalian cells on illuminating phenothiazinium dyes with red light. Suitable dyes include toluidine blue, used in cancer diagnosis because of localization in tumors, and methylene blue, used in experimental photodynamic therapy of cancer. The photosensitized oxidation of indole acetic acid forms a free...

The "bound auxin" of Zea mays, first described by Berger and Avery (Amer. J. Bot. 1944; 31: 199-203) has been purified and partially characterized. It is an indole-3-acetic acid-containing, high molecular weight, lipophilic cellulosicglucan. The indole-3-acetic acid is in ester linkage as evidenced by indoleacetamide formation upon ammonolysis. The glucan is of variable chain length and compris...

Recent studies applying Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) showed that the exometabolome of marine bacteria is composed of a surprisingly high molecular diversity. To shed more light on how this diversity is generated we examined the exometabolome of two model strains of the Roseobacter group, Phaeobacter inhibens and Dinoroseobacter shibae, grown on glutama...

Indole-3-acetic acid (IAA) homeostasis was investigated during seed germination and early seedling growth in Scots pine (Pinus sylvestris). IAA-ester conjugates were initially hydrolyzed in the seed to yield a peak of free IAA prior to initiation of root elongation. Developmental regulation of IAA synthesis was observed, with tryptophan-dependent synthesis being initiated around 4 d and tryptop...

The suggestion that indole-3-acetic acid (IAA)-stimulated ethylene production is associated with oxidative degradation of IAA and is mediated by 3-methyleneoxindole (MOI) has been tested in mung bean (Phaseolus aureus Roxb.) hypocotyl segments. While IAA actively stimulated ethylene production, MOI and indole-3-aldehyde, the major products of IAA oxidation, were inactive. Tissues treated with a...