OBJECTIVE To compare direct and indirect bead-based T-cell isolation followed by magnetic bead-based DNA isolation. METHODS T-cells were isolated by direct or indirect selection with magnetic bead coated antbiodies followed by magnetic bead-based automated DNA isolation in 10 healthy subjects. Purity of T-cells, purity of DNA (by A260/A280 ratio measurement) and DNA concentration were assesse...

sainfoin (onobrychis viciifolia scop.) is a perennial forage legume has recognized for its feed value and high stability. this experiment was conducted to investigate the relationship among morphological and agronomic traits using multivariate statistical methods including correlation, regression and factor analysis. twenty one accessions of sainfoin were evaluated in a randomized complete bloc...

a research study was carried out on the indirect selection efforts amongst small scale holders of west african dwarf (wad) goats based on coat pigmentation and wattle types in ogbomoso, nigeria using personal interviews and structured questionnaires given using simple random on-farm techniques. the questionnaires were focused on the ownership distribution, preferred pigmentation and wattle type...

BACKGROUND AND OBJECTIVES Health selection into unemployment may be either direct or operate by reference to health-related behaviours rather than health per se (indirect selection). Panel data are desirable to investigate selection effects, and the two types of selection processes may be concurrent. We examine jointly the roles of health and health-related behaviours as precursors of unemploym...

seed cap tightness is known to be highly associated with the low germinability of sugar beet fruits. despite, accomplishment of physiological phases of germination in germinating sugar beet seed with a tight cap, seedling emergence is usually suppress by the cap. as the seed coat (including the cap) is originated from ovary wall of the mother plant, seed cap tightness could be considered as one...

While much has been written describing biodiversity, its global decline, and the need for action, the scientific underpinnings guiding conservation practice have received little attention. We surveyed 10 large-scale forest management plans in the U.S. to establish which ecological concepts are commonly used to guide forest biodiversity conservation and evaluate the relative importance of these ...

This paper advances our understanding of why some transactions are “embedded” in preexisting social relations and some are not. While prior research tends to interpret such relations as instruments that transaction parties use to control one another in situations of vulnerability, this interpretation has difficulty accounting for high rates of embeddedness among the many consumer transactions w...

Traditional quantitative genetics assumes that an individual's phenotype is determined by both genetic and environmental factors. For many animals, part of the environment is social and provided by parents and other interacting partners. When expression of genes in social partners affects trait expression in a focal individual, indirect genetic effects occur. In this study, we explore the effec...