Giardiasis is endemic in all regions of the world. Giardia lamblia (G. lamblia) cysts are spread in Egypt via the fecal-oral route, through ingestion of the cyst with contaminated food or water. The symptoms of giardiasis vary from the asymptomatic passage of cysts to chronic diarrhea, malabsorption and weight loss. Although microscopy has the advantage of low cost and ability to simultaneously...

Background: Platanus species are widely cultured around the world and considered an important cause of allergic reactions. In the present study, we developed a sandwich ELISA to quantify Pla or 3 allergen in P. orientalis pollen extracts grown near high-traffic roads and compared it to pollen extracts collected from rural areas as control. Methods: Pollen samples were collected from three po...

Plant diseases caused by viruses are the most menace to sustainable agriculture, leading numerous billion dollars in losses annually. Proper and accurate detection of plant is always key developing appropriate solutions manage economic them. Advances technology have simplified tools available detect diagnose at a time when control measures should be used. Currently, nucleic acid serological bas...

Diagnosis of larval migrans (LM) is usually done by immunodiagnostic methods. These methods, however, simply show the presence or absence of antibody but not the active infection of the patients. Therefore, we aimed to establish a diagnostic method for detecting circulating Toxocara canis antigen using a sandwich, ELISA. Monoclonal antibodies (MAb) were produced against the excretory-secretory ...

this study was conducted in khorasan-razavi during 23 months (since february 2004 to december 2005). the samples (83 samples) of suspected farms were collected from tongue and mouth epithelium and tested by indirect sandwich elisa and rt-pcr. during the study, twelve foci during the year 2004 and 23 foci during the year 2005 (up to december) were approved. maximum distribution of fmd foci was o...

Foot-and-mouth disease virus (FMDV) continues to be a significant economic problem worldwide. Control of the disease involves the use of killed-virus vaccines, a control measure developed decades ago. After natural infection, the primary site of replication of FMDV is the pharyngeal area, suggesting that a mucosal immune response is the most effective. Humoral immunity to killed-virus vaccinati...

An enzyme-linked immunosorbent assay (ELISA) was developed for the rapid and simple differentiation of toxigenic from nontoxigenic strains of Pasteurella multocida. The sandwich ELISA is based on two different murine monoclonal antibodies with specificity for the P. multocida toxin. The ELISA, which is now used as a routine test in Denmark, has several advantages compared with previously descri...

Adducts of benzo[a]pyrene-diolepoxide (BPDE) with blood nucleophiles have been used as biomarkers of exposure to polycyclic aromatic hydrocarbons (PAHs). The most popular such assay is a competitive enzyme-linked immunosorbent assay (ELISA) that employs monoclonal antibody 8E11 to detect benzo[a]pyrene tetrols following hydrolysis of BPDE adducts from lymphocyte DNA or human serum albumin (HSA)...