نتایج جستجو برای: indirect plate elisa

تعداد نتایج: 195615  

فلاحتی, مهربان, اخلاقی, لامع, حیدری کهن, فریبا , زینی, فریده, شریفی سرخه‌ریزی, مینا, شریفی نیا, سمیه, غلمان, محسن,

  Background : Vulvovaginal candidiasis occurs due to the overgrowth of candida in genital system mucosa of females.   Symptoms and signs of vulvovaginal candidiasis are unspecific, therefore its diagnosis based on them (symptoms and signs) is not certain.   The aim of this research was comparison the result of indirect immunofluorescence and ELISA with culture and direct microscopy examination...

2017
Rafael Pandolfi Denise Ramos de Almeida Marcelo Alves Pinto Luiz Carlos Kreutz Rafael Frandoloso

Hepatitis E Virus (HEV) is a zoonotic pathogen responsible for causing acute hepatitis in human, especially in developing countries. Diagnosis of HEV usually relies on the detection of antibodies mostly by enzyme-linked immunosorbent assay (ELISA). In the present study, we designed a new indirect ELISA (iELISA) based on a short recombinant peptide derived from the capsid protein (ORF2p) and dem...

Journal: :Journal of animal science 1996
M E Doumit S M Lonergan J R Arbona J Killefer M Koohmaraie

An indirect antibody ELISA was developed for rapid and sensitive quantification of skeletal muscle calpastatin. Polyclonal antibodies were raised in rabbits against recombinant calpastatin, corresponding to domains 2, 3, and 4 of bovine skeletal muscle calpastatin. Western blot analysis revealed that these antibodies specifically recognize an immunoreactive calpastatin protein of approximately ...

1996
M. E. Doumit S. M. Lonergan J. R. Arbona M. Koohmaraie Roman L. Hruska

An indirect antibody ELISA was developed for rapid and sensitive quantification of skeletal muscle calpastatin. Polyclonal antibodies were raised in rabbits against recombinant calpastatin, corresponding to domains 2, 3, and 4 of bovine skeletal muscle calpastatin. Western blot analysis revealed that these antibodies specifically recognize an immunoreactive calpastatin protein of approximately ...

2018
J Awah-Ndukum M M M Mouiche H N Bayang V Ngu Ngwa E Assana K J M Feussom T K Manchang P A Zoli

A cross-sectional seroprevalence study was conducted on cattle in the North and Adamawa Regions of Cameroon to investigate the status of bovine brucellosis and identify potential risk factors. The diagnosis was carried out using the Rose Bengal Plate test (RBPT) and indirect ELISA (i-ELISA), while questionnaires were used to evaluate risk factors for bovine brucellosis in cattle. The Bayesian a...

2016
Luis G. Giménez-Lirola Lina Mur Belen Rivera Mark Mogler Yaxuan Sun Sergio Lizano Christa Goodell D. L. Hank Harris Raymond R. R. Rowland Carmina Gallardo José Manuel Sánchez-Vizcaíno Jeff Zimmerman

In the absence of effective vaccine(s), control of African swine fever caused by African swine fever virus (ASFV) must be based on early, efficient, cost-effective detection and strict control and elimination strategies. For this purpose, we developed an indirect ELISA capable of detecting ASFV antibodies in either serum or oral fluid specimens. The recombinant protein used in the ELISA was sel...

Journal: :Applied and environmental microbiology 1988
H A Kemp D B Archer M R Morgan

Three microtitration plate enzyme-linked immunosorbent assays (ELISAs) have been developed: a competitive ELISA and a two-site (or indirect sandwich) ELISA for Methanosarcina mazei S6 and a two-site ELISA for Methanobacterium bryantii FR-2. The assays were sensitive, with limits of cell protein detection of 3 ng ml, 5 ng ml, and 50 ng ml, respectively, and showed good precision. The M. mazei as...

2002
Yoo Jung Kim Young Ae Cho Hye-Sung Lee Yong Tae Lee Shirley J. Gee Bruce D. Hammock

The synthetic method for haptens of organophosphorus (OP) pesticides with a spacer arm (amino carboxylic acid) attached at the pesticide thiophosphate group was simplified to a large extent. While the previous synthetic approach for this type of haptens requires seven steps, the present process involves only two steps. Using this process, five haptens of fenthion differing in spacer arm length ...

Journal: :Journal of analytical toxicology 2002
Tom Kupiec Lacinda DeCicco Vina Spiehler Gary Sneed Philip Kemp

The object of this study was to evaluate the suitability of the Neogen Corp. microtiter plate enzyme-linked immunoassays (ELISA) for the screening of postmortem blood for amphetamine and methamphetamine and to choose the more appropriate assay for screening. Forty-seven postmortem whole blood specimens were obtained from drug-involved deaths, which had been screened and confirmed positive for m...

2017
Niranjan Kumar Anju Varghese J. B. Solanki

Aim The objective of the present study was to know the seroprevalence status of Fasciola gigantica infection in cattle and buffaloes using cysteine proteinase (CP) antigen in dot enzyme-linked immunosorbent assay (ELISA) format under field conditions. Materials and Methods As per the standard protocol, the sera were collected from the blood of 112 cattle and 38 buffaloes of coastal areas of N...

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