The chemical shift sensitivity and significant signal enhancement afforded by laser-polarized 129Xe have motivated the application of 129Xe NMR to biological imaging and spectroscopy. Recent research done by our group has used laser-polarized 129Xe in biomolecular assays that detect ligand-binding events and distinguish protein conformations. The successful application of unfunctionalized and f...

RNA interference (RNAi), an effective technique for regulating or silencing specific genes, can be applied to treat various diseases. Multiple clinical trials using RNAi are ongoing, and molecular imaging can serve as a powerful tool in RNAi-based therapies. This brief review will highlight the current progress on in vivo imaging of RNAi delivery and silencing effects. Incorporation of suitable...

The molecular charge of fluorescent bioprobes has recently received much attention due to its influence on cellular uptake. Herein, we demonstrate the effect of the molecular charge of glucose bioprobes on their GLUT-specific cellular uptake. We also applied GB2-Cy3 to in vivo imaging in the zebrafish model.

Indocyanine green (ICG) is widely used in medical imaging and testing. Its complex spectral behavior and low quantum yield limits some applications. We show that proximity of ICG to a metallic silver particle increases its intensity approximately 20-fold and decreases the decay time. Since the rate of photobleaching is not increased, our results suggest that ICG-silver particle complexes can yi...

The combination of two-photon in vivo imaging and genetic labeling of specific cell types in the mouse brain is a powerful method to refine our understanding of brain circuitry and to dissect the contribution of specific neural classes to cortical function. The synthetic calcium indicators are the best fluorescent reporters for cellular activity that are presently available but their spectral p...

Cellular in vivo imaging can reveal the induction, progression and resolution of nervous system pathology in real-time. In this review, we summarize recent research in the field to illustrate how in vivo imaging has enhanced our understanding of a variety of neurological conditions caused by trauma, degeneration, ischemia or inflammation. Finally, we look ahead and discuss how novel biosensors ...

The ultimate check of the actual dose delivered to a patient in radiotherapy can only be achieved by using in vivo dosimetry. This work reports a pilot study to test the applicability of a thermoluminescent dosimetric system for performing in vivo entrance dose measurements in external photon beam radiotherapy. The measurements demonstrated the value of thermoluminescent dosimetry as a treatmen...

Twenty-four patients of moderate persistent perennial asthma with documented aggravation to severe persistent asthma, during monsoon season in the past two years, were put on specific elimination diet during August and September. The diet was based on results of in-vitro allergy tests for a selected food panel. On specific elimination diet, five patients improved to mild persistent asthma and t...

Inhibitory neurons are known to play a vital role in defining the window for critical period plasticity during development, and it is increasingly apparent that they continue to exert powerful control over experience-dependent cortical plasticity in adulthood. Recent in vivo imaging studies demonstrate that long-term plasticity of inhibitory circuits is manifested at an anatomical level. Change...

An interferometric method for parallel optical spectroscopy in the kilohertz range is reported, as well as its experimental validation in the context of high-speed laser Doppler imaging in vivo. The interferometric approach enables imaging in the low light conditions of a 2 kHz frame rate recording with a complementary metal-oxide semiconductor camera. Observation of mice craniums with near-inf...