Fluorescence in situ hybridization (FISH) utilizes fluorescent probes to bind portions of DNA that have a high degree of sequence complementarity. This allows researchers to detect and localize specific DNA sequences on chromosomes or RNA targets in various cell and tissue types to determine the spatial-temporal patterns of gene expression within. In medicine, FISH can be used to diagnose or ev...

in-situ testing techniques have proven to be successful in improving the speed and reliability of geotechnical investigations. one of the most common in-situ methods in engineering geology and site investigation is cone penetration test (cpt), which is mainly used for characterization of soils, as it is a robust, simple, fast, reliable and economic test that can provide continuous soundings of ...

Whole-mount in situ hybridization (WISH) is a powerful tool for visualizing gene expression patterns in specific cell and tissue types. Each model organism presents its own unique set of challenges for achieving robust and reproducible staining with cellular resolution. Here, we describe a formaldehyde-based WISH method for the freshwater planarian Schmidtea mediterranea developed by systematic...

In recent years, the diagnostic capacity of colposcopy has been called into question. Recent studies have indicated that the sensitivity of this method for the detection of cervical intraepithelial neoplasia 3 (CIN 3) is between 54% and 85% [1]. In additional, it has been reported that biopsies performed under colposcopy guidance detects only two-thirds of CIN 3 lesions [2], and several researc...