A number of parameters affecting the adsorption of rRNA and poly(A)-containing RNA to Millipore filters were investigated separately. Binding of both types of RNA to the filter was dependent on the concentration of RNA, pH and Mg2+ concentration of the reaction mixture. Both types of RNA bound to the filter optimally at slightly acid pH values. The binding of poly(A)-containing RNA to the filte...

Alkyl sulfate monoesters are involved in cell signaling and structure. Alkyl sulfates are also present in many commercial detergents. Here, we show that monomethyl sulfate acts as an efficient alkylating agent in water, reacting spontaneously with oxygen nucleophiles >100-fold more rapidly than do alkylsulfonium ions, the usual methyl donors in living organisms. These reactions of methyl sulfat...

or ferricyanide were not able to replace ascorbate and induce lipid peroxide formation. 2. The rate of ascorbate-induced peroxidation is optimum at pH 6-0 whereas the rate of the NADPH system is optimum at pH7-0. Both systems require phosphate for maximum activity. 3. Lipid peroxide formation occurs at the maximum specific rate in very dilute microsome suspensions (0-15mg. of protein/ ml.). 4. ...

A flow injection method has been developed for the direct determination of free available Pb(II). The method is based on the chemical sorption of Pb(II), from pH7 solutions, on a column packed of chelating resin. The retained complex was afterwards eluted with hydrochloric acid followed by hydride generation with reduction by tetrahydroborate. The preconcentration system proposed in this paper ...

The adsorption of phosphorus (P) onto three industrial solid wastes (fly ash, red mud and ferric-alum water treatment residual (FAR)) and their modified materials was studied systematically via batch experiments. Compared with two natural adsorbents (zeolite and diatomite), three solid wastes possessed a higher adsorption capacity for P because of the higher Fe, Al and Ca contents. After modifi...